ACOX1 Monoklonaler Antikörper

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• KD/KO Validated

ACOX1 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

CloneNo.

3E12E10

Kat-Nr. : 68017-1-Ig

Synonyme

ACOX, ACOX1, AOX, PALMCOX, Palmitoyl CoA oxidase, SCOX

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Galerie der Validierungsdaten

• 

  WB analysis using 68017-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68017-1-Ig (ACOX1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis of rat liver using 68017-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 68017-1-Ig (ACOX1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 68017-1-Ig

  WB result of ACOX1 antibody (68017-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACOX1 transfected HepG2 cells.

• 

  WB analysis of pig liver using 68017-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 68017-1-Ig (ACOX1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, Hausschwein-Lebergewebe, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, NIH/3T3-Zellen, Rattenlebergewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

68017-1-Ig bindet in WB, IF, ELISA ACOX1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ACOX1 fusion protein Ag28037
• Vollständiger Name: acyl-Coenzyme A oxidase 1, palmitoyl
• Berechnetes Molekulargewicht: 74 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC010425
• Gene symbol: ACOX1
• Gene ID (NCBI): 51
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ACOX1(Peroxisomal acyl-coenzyme A oxidase 1) is the first and rate-limiting enzyme in the peroxisomal fatty acid beta-oxidation pathway and catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. It belongs to the acyl-CoA oxidase family and also named as ACOX, AOX, SCOX. It has 3 isoforms produced by alternative splicing with the molecular mass of 70-74 kDa and can be cleaved post-translationally into a 50-kDa and a 22-kDa subunit between Val468 and Ala469 (PMID: 10672038). Defects in ACOX1 are the cause of adrenoleukodystrophy pseudoneonatal (Pseudo-NALD).

Publikationen

Species	Application	Title
rat	WB,IF	Phytomedicine; An integrated network pharmacology and cell metabolomics approach to reveal the role of rhein, a novel PPARα agonist, against renal fibrosis by activating the PPARα-CPT1A axis.; Authors - Qiming Xiao
rat	WB	Front Pharmacol; Lipidomics reveals the potential mechanism of honokiol against adenine-induced chronic kidney disease; Authors - Xinhui Liu