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ASIC2 Polyklonaler Antikörper

ASIC2 Polyklonal Antikörper für IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17851-1-AP

Synonyme

ACCN, ACCN1, Acid sensing ion channel 2, ASIC2, ASIC2a, BNaC1, BNC1, Brain sodium channel 1, hBNaC1, Mammalian degenerin homolog, MDEG

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of rat brain tissue using ASIC2 Polyclonal antibody (17851-1-AP)

    WB analysis of rat brain using 17851-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 17851-1-AP (ASIC2 antibody at dilution of 1:300 incubated at 4 degree celsius over night.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using ASIC2 Polyclonal antibody (17851-1-AP)

    IP experiment of mouse brain using 17851-1-AP

    IP Result of anti-ASIC2 (IP:17851-1-AP, 4ug; Detection:17851-1-AP 1:500) with mouse brain tissue lysate 4000ug.

Geprüfte Anwendungen

Erfolgreiche Detektion in WBRattenhirngewebe
Erfolgreiche IPMaushirngewebe

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:200-1:1000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 2 publications below

Produktinformation

17851-1-AP bindet in WB, IP,ELISA ASIC2 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen ASIC2 fusion protein Ag12301
Vollständiger Name amiloride-sensitive cation channel 1, neuronal
Berechnetes Molekulargewicht 512 aa, 58 kDa
Beobachtetes Molekulargewicht 70 kDa
GenBank-ZugangsnummerBC075043
Gene symbol ACCN1
Gene ID (NCBI) 40
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ASIC2 (acid-sensing ion channel 2), also known as ACCN1 (amiloride-sensitive cation channel 1, neuronal), is a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. ASIC2 is widely expressed in the central nervous system (CNS), may playing a role in neurotransmission. It has two alternatively spliced variants, ASIC2a and ASIC2b.

Protokolle

Produktspezifische Protokolle
WB protocol for ASIC2 antibody 17851-1-APProtokoll herunterladen
IP protocol for ASIC2 antibody 17851-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Aging (Albany NY)

ASIC1 and ASIC3 mediate cellular senescence of human nucleus pulposus mesenchymal stem cells during intervertebral disc degeneration.

Authors - Jingyu Ding
View Article
humanWB

Sci Rep

Syntabulin regulates the trafficking of PICK1-containing vesicles in neurons.

Authors - Junyu Xu
View Article