ACAT2 Monoklonaler Antikörper

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• KD/KO Validated

ACAT2 Monoklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Kaninchen, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

1F5F11

Kat-Nr. : 68005-1-Ig

Synonyme

ACAT2, ACTL

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Galerie der Validierungsdaten

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  WB analysis using 68005-1-Ig

  Various cell lysates were subjected to SDS PAGE followed by western blot with 68005-1-Ig (ACAT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of HepG2 using 68005-1-Ig

  WB result of ACAT2 antibody (68005-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACAT2 transfected HepG2 cells.

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  WB analysis using 68005-1-Ig

  Brain tissues were subjected to SDS PAGE followed by western blot with 68005-1-Ig (ACAT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HCT 116 using 68005-1-Ig

  HCT 116 cells were subjected to SDS PAGE followed by western blot with 68005-1-Ig (ACAT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 68005-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ACAT2 antibody (68005-1-Ig, Clone: 1F5F11 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HCT 116-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, Kaninchenhirngewebe, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF): IF : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68005-1-Ig bindet in WB, IF, ELISA ACAT2 und zeigt Reaktivität mit human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ACAT2 fusion protein Ag6596
• Vollständiger Name: acetyl-Coenzyme A acetyltransferase 2
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC000408
• Gene symbol: ACAT2
• Gene ID (NCBI): 39
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.