ACADL Polyklonaler Antikörper

ACADL Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 17442-1-AP

Synonyme

ACAD4, ACADL, LCAD

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Galerie der Validierungsdaten

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  WB analysis using 17442-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17442-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 17442-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17442-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17442-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 17442-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 17442-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 17442-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 17442-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 17442-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 17442-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 17442-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 17442-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17442-1-AP (ACADL antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 17442-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17442-1-AP (ACADL antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 17442-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 17442-1-AP (ACADL Antibody) at dilution of 1:50.

• 

  IHC staining of human liver using 17442-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 17442-1-AP (ACADL Antibody) at dilution of 1:50.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, NIH/3T3-Zellen, Rattennierengewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:100-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

17442-1-AP bindet in WB, IHC, ELISA ACADL und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ACADL fusion protein Ag11530
• Vollständiger Name: acyl-Coenzyme A dehydrogenase, long chain
• Berechnetes Molekulargewicht: 430 aa, 48 kDa
• Beobachtetes Molekulargewicht: 45-48 kDa
• GenBank-Zugangsnummer: BC039063
• Gene symbol: ACADL
• Gene ID (NCBI): 33
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ACADL(Long-chain specific acyl-CoA dehydrogenase, mitochondrial) is also named as LCAD and belongs to the acyl-CoA dehydrogenase family.It catalyzes the first reaction of the mitochondrial β-oxidation of fatty acids and is synthesized in the cytosol as a precursor that is larger than its mature from. The size difference between the precursor and mature rat LCAD has been estimated to be 3 kDa on the basis of SDS-PAGE(PMID:1774065).Defects in ACADL are a cause of acyl-CoA dehydrogenase very long-chain deficiency (ACADVLD).

Publikationen

Species	Application	Title
human	WB	Nat Commun; Enoyl-CoA hydratase-1 regulates mTOR signaling and apoptosis by sensing nutrients.; Authors - Ya-Kun Zhang
rat	WB	Front Pharmacol; Lipidomics reveals the potential mechanism of honokiol against adenine-induced chronic kidney disease; Authors - Xinhui Liu
mouse	WB	Front Pharmacol; The serum acylcarnitines profile in epileptic children treated with valproic acid and the protective roles of peroxisome proliferator-activated receptor a activation in valproic acid-induced liver injury; Authors - Yiyi Ma
human,mouse	WB	Diabetologia; Chaperonin counteracts diet-induced non-alcoholic fatty liver disease by aiding sirtuin 3 in the control of fatty acid oxidation; Authors - Shao-Wen Weng