ABI2 Polyklonaler Antikörper

ABI2 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 14890-1-AP

Synonyme

Abelson interactor 2, ABI 2, ABI2, ABI2B, Abl binding protein 3, abl interactor 2, AblBP3, AIP 1, Arg binding protein 1, ArgBP1, argBPIA, argBPIB, SSH3BP2

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Galerie der Validierungsdaten

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  WB analysis using 14890-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14890-1-AP (ABI2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 14890-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14890-1-AP (ABI2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse uterus using 14890-1-AP

  mouse uterus tissue were subjected to SDS PAGE followed by western blot with 14890-1-AP (ABI2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 14890-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14890-1-AP (ABI2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 14890-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14890-1-AP (ABI2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 14890-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14890-1-AP (ABI2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 14890-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14890-1-AP (ABI2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 14890-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14890-1-AP (ABI2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 14890-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14890-1-AP (ABI2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 14890-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14890-1-AP (ABI2 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, K-562-Zellen, Mausherzgewebe, Maushirngewebe, Maushodengewebe, Maus-Uterusgewebe, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:6000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• CoIP: See 1 publications below

Produktinformation

14890-1-AP bindet in WB, IHC, CoIP, ELISA ABI2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ABI2 fusion protein Ag6679
• Vollständiger Name: abl interactor 2
• Berechnetes Molekulargewicht: 56 kDa
• Beobachtetes Molekulargewicht: 52-56 kDa
• GenBank-Zugangsnummer: BC001439
• Gene symbol: ABI2
• Gene ID (NCBI): 10152
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,CoIP	Nat Commun; GRAF1 integrates PINK1-Parkin signaling and actin dynamics to mediate cardiac mitochondrial homeostasis; Authors - Qiang Zhu
mouse,human	WB	Nat Commun; hnRNP A1 dysfunction alters RNA splicing and drives neurodegeneration in multiple sclerosis (MS); Authors - Hannah E Salapa
human	WB	Nat Cell Biol; Actin cytoskeleton vulnerability to disulfide stress mediates disulfidptosis; Authors - Xiaoguang Liu