ABCG5 Polyklonaler Antikörper

ABCG5 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

24

Kat-Nr. : 27722-1-AP

Synonyme

ABCG5, Sterolin 1, STSL

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Galerie der Validierungsdaten

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  WB analysis using 27722-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27722-1-AP (ABCG5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27722-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27722-1-AP (ABCG5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 27722-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 27722-1-AP (ABCG5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 27722-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 27722-1-AP (ABCG5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 27722-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 27722-1-AP (ABCG5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 27722-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27722-1-AP (ABCG5 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 27722-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27722-1-AP (ABCG5 antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Caco-2-Zellen, HepG2-Zellen, Maus-Kolongewebe, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 24 publications below

Produktinformation

27722-1-AP bindet in WB, IHC, ELISA ABCG5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ABCG5 fusion protein Ag26844
• Vollständiger Name: ATP-binding cassette, sub-family G (WHITE), member 5
• Berechnetes Molekulargewicht: 72 kDa
• Beobachtetes Molekulargewicht: 68 kDa~72 kDa
• GenBank-Zugangsnummer: NM_022436
• Gene symbol: ABCG5
• Gene ID (NCBI): 64240
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Diabetes; Long Noncoding RNA lncRHL Regulates Hepatic VLDL Secretion by Modulating hnRNPU/ BMAL1/MTTP Axis.; Authors - Xuan Shen
human	WB	Cell Mol Gastroenterol Hepatol; A Mitochondrial DNA Variant Elevates the Risk of Gallstone Disease by Altering Mitochondrial Function.; Authors - Dayan Sun
human	WB	Mol Ther Nucleic Acids; HFD-induced TRAF6 upregulation promotes liver cholesterol accumulation and fatty liver development via EZH2-mediated miR-429/PPARα axis.; Authors - Zhi Zhang
human	WB	J Cell Mol Med; The marine-derived furanone reduces intracellular lipid accumulation in vitro by targeting LXRα and PPARα.; Authors - Ting Li
rat	WB	Front Nutr; Preparation of microgel co-loaded with nuciferine and epigallocatechin-3-gallate for the regulation of lipid metabolism; Authors - Shengnan Zhu
mouse,human	WB	Biochem Pharmacol; Resveratrol enhances trans-intestinal cholesterol excretion through selective activation of intestinal liver X receptor alpha.; Authors - Juan Pang