ABCB6 Polyklonaler Antikörper

ABCB6 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14996-1-AP

Synonyme

ABC, ABC14, ABCB6, EST45597, Mt ABC transporter 3, MTABC3, P glycoprotein related protein, PRP, umat

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 14996-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14996-1-AP (ABCB6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 14996-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14996-1-AP (ABCB6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 14996-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14996-1-AP (ABCB6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 14996-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14996-1-AP (ABCB6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 14996-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14996-1-AP (ABCB6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 14996-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14996-1-AP (ABCB6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 14996-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14996-1-AP (ABCB6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 14996-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14996-1-AP (ABCB6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

14996-1-AP bindet in WB, IHC, ELISA ABCB6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ABCB6 fusion protein Ag6982
• Vollständiger Name: ATP-binding cassette, sub-family B (MDR/TAP), member 6
• Berechnetes Molekulargewicht: 94 kDa
• Beobachtetes Molekulargewicht: 170 kDa
• GenBank-Zugangsnummer: BC000559
• Gene symbol: ABCB6
• Gene ID (NCBI): 10058
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.