AATF Monoklonaler Antikörper

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• KD/KO Validated

AATF Monoklonal Antikörper für FC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

CloneNo.

2A6G6

Kat-Nr. : 67868-1-Ig

Synonyme

AATF, CHE 1, CHE1, DED, Protein AATF, Rb binding protein Che 1, Traube

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Galerie der Validierungsdaten

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  WB analysis using 67868-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67868-1-Ig (AATF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of Neuro-2a using 67868-1-Ig

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 67868-1-Ig (AATF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 67868-1-Ig

  WB result of AATF antibody (67868-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AATF transfected HepG2 cells.

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  FC experiment of HeLa using 67868-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human AATF (67868-1-Ig, Clone:2A6G6) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 (KFA022), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HCT 116-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, LNCaP-Zellen, Neuro-2a-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Durchflusszytometrie (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 2 publications below

Produktinformation

67868-1-Ig bindet in WB, IHC, FC, ELISA AATF und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: AATF fusion protein Ag0195
• Vollständiger Name: apoptosis antagonizing transcription factor
• Berechnetes Molekulargewicht: 63 kDa
• Beobachtetes Molekulargewicht: 80-90 kDa
• GenBank-Zugangsnummer: BC000591
• Gene symbol: AATF
• Gene ID (NCBI): 26574
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,IHC	Onco Targets Ther; AATF is Overexpressed in Human Head and Neck Squamous Cell Carcinoma and Regulates STAT3/Survivin Signaling; Authors - Lin Fu
human	WB,IHC	Onco Targets Ther; AATF is Overexpressed in Human Bladder Cancer and Regulates Chemo-Sensitivity Through Survivin.; Authors - Shutao Tan