14-3-3E Monoklonaler Antikörper

14-3-3E Monoklonal Antikörper für WB,ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

2

CloneNo.

3A3A4

Kat-Nr. : 66946-1-Ig

Synonyme

14 3 3 protein epsilon, 14 3 3E, FLJ45465, KCIP 1, MDCR, MDS, YWHAE

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Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 66946-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66946-1-Ig (14-3-3E antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66946-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66946-1-Ig (14-3-3E antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 66946-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66946-1-Ig (14-3-3E antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66946-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66946-1-Ig (14-3-3E antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 66946-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66946-1-Ig (14-3-3E antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 66946-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66946-1-Ig (14-3-3E antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 66946-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66946-1-Ig (14-3-3E antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, Jurkat-Zellen, MCF-7-Zellen, NIH/3T3-Zellen, RAW 264.7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

66946-1-Ig bindet in WB, ELISA 14-3-3E und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: 14-3-3E fusion protein Ag25186
• Vollständiger Name: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide
• Berechnetes Molekulargewicht: 255 aa, 29 kDa
• Beobachtetes Molekulargewicht: 28-32 kDa
• GenBank-Zugangsnummer: BC000179
• Gene symbol: YWHAE
• Gene ID (NCBI): 7531
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

14-3-3 epsilon (also known as YWHAE) is a member of 14-3-3 proteins which were the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis.

Publikationen

Species	Application	Title
human	WB	Cancer Manag Res; miR-31-5p Regulates 14-3-3 ɛ to Inhibit Prostate Cancer 22RV1 Cell Survival and Proliferation via PI3K/AKT/Bcl-2 Signaling Pathway.; Authors - Jiafu Zhao
mouse		Oncol Lett; miR-29b-3p inhibits 22Rv1 prostate cancer cell proliferation through the YWHAE/BCL-2 regulatory axis.; Authors - Jiafu Zhao