p53 antibody (pAb)
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
WB
Cat No : 61657,61658 61657
Synonyms
Validation Data Gallery
Product Information
| Tested Applications |
WB
Applications Validated by Active Motif: WB*: 1:500 - 1:2,000 dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot. For p53, we also offer AbFlex p53 Recombinant Antibody (rAb). For details, see Catalog No. 91247. |
| Tested Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | This antibody was raised against recombinant human p53 protein. |
| Full Name | p53 antibody (pAb) |
| Synonyms | TP53, p53, Tumor Protein P53, TRP53, P53, Cellular Tumor Antigen P53, Antigen NY-CO-13, Mutant Tumor Protein 53, Phosphoprotein P53, P53 Tumor Suppressor, BCC7, Transformation-Related Protein 53, LFS1, Tumor Protein 53, Li-Fraumeni Syndrome1, Tumor Suppressor P53, polyclonal, pab, antibody, antibodies, western blotting, wb, sample |
| Molecular weight | 53 kDa |
| GenBank accession number | NP_000537 |
| RRID | AB_2793723 |
| Purification Method | Affinity Purified |
| Buffer | Purified IgG in PBS with 50% glycerol and 0.02% sodium azide. Sodium azide is highly toxic. |
| Storage | Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage. |
Background Information
p53 is the most important tumor suppressor in the genome. It is responsive to numerous genotoxic stresses, which activates its transcription factor activity, in turn causing cell-cycle arrest by activating expression of p21 Cip/WAF. Mutant p53 that has lost its DNA-binding function interferes with the activity of native p53 and leads to oncogenic transformation. Alternatively, transformation may be caused by overexpression of Mdm2/Hdm2, a ubiquitin ligase specific for p53, which causes its destabilization. Inactivation of p53 is often coincident with hyperactivation of NFkB (NFkB p50 and NFkB p65), both of which serve to inhibit apoptosis.