• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

p53 antibody (mAb) (Clone DO1)

Host / Isotype

Mouse / IgG

Reactivity

Human

Applications

ChIP, IP, WB

CloneNo.

DO1

Activ Motif logo

Cat No : 39553,39554 39553

Synonyms

p53, p 53, clone DO1, DO1, mAb, monoclonal, clone DO1, antibody, antibodies, sample, dbdabs

Validation Data Gallery

  • p53 mAb (Clone DO1) tested by Immunoprecipitation. 5 ug of p53 mAb (Clone DO1) was used to immunoprecipitate p53 from 500 ug of camptothecin-treated U2OS nuclear extract (lane 2). 5 ug of mouse IgG was also used as a control (lane 1). The immunoprecipitated protein was detected by western blotting using the same p53 antibody.

    p53 mAb (Clone DO1) tested by Immunoprecipitation. 5 ug of p53 mAb (Clone DO1) was used to immunoprecipitate p53 from 500 ug of camptothecin-treated U2OS nuclear extract (lane 2). 5 ug of mouse IgG was also used as a control (lane 1). The immunoprecipitated protein was detected by western blotting using the same p53 antibody.

  • p53 mAb (Clone DO1) tested by Western blot. Western Blot: Nuclear extract of U2OS cells (20 μg) probed with p53 mAb (Clone DO1) (1 ug/ml). Lane 1: No treatment. Lane 2: Cells treated with camptothecin.

    p53 mAb (Clone DO1) tested by Western blot. Western Blot: Nuclear extract of U2OS cells (20 μg) probed with p53 mAb (Clone DO1) (1 ug/ml). Lane 1: No treatment. Lane 2: Cells treated with camptothecin.

Product Information

Tested Applications ChIP, IP, WB

Applications Validated by Active Motif: IP: 5 ug per IP WB: 0.5 - 2 ug/ml dilution This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91247.
Tested Reactivity Human
Host / Isotype Mouse / IgG
Class Monoclonal
Type Antibody
Immunogen This p53 antibody was raised against full-length recombinant human p53 protein.
Full Name p53 antibody (mAb) (Clone DO1)
Synonyms p53, p 53, clone DO1, DO1, mAb, monoclonal, clone DO1, antibody, antibodies, sample, dbdabs
Molecular weight 53 kDa
GenBank accession numberNP_000537
RRIDAB_2793254
Purification Method Protein G Chromatography
Buffer Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

p53 is the most important tumor suppressor in the genome. It is responsive to numerous genotoxic stresses, which activates its transcription factor activity, in turn causing cell-cycle arrest by activating expression of p21 Cip/WAF. Mutant p53 that has lost its DNA-binding function interferes with the activity of native p53 and leads to oncogenic transformation. Alternatively, transformation may be caused by overexpression of Mdm2/Hdm2, a ubiquitin ligase specific for p53, which causes its destabilization. Inactivation of p53 is often coincident with hyperactivation of NFkB (NFkB p50 and NFkB p65), both of which serve to inhibit apoptosis.