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p300 antibody (mAb) (Clone NM11)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

ChIP, ChIP-Seq, ICC, IF, IP, WB

CloneNo.

NM11

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Cat No : 61401,61402,61903 61401

Synonyms

p300, E1A binding protein p300, RSTS2, KAT3B, E1A-binding protein, 300kD, histone acetyltransferase p300, E1A-associated protein p300, P300, p300 HAT, antibody, monoclonal, mAb, immunoprecipitation, ip, immunofluorescence, if, western blotting, wb, sample

Validation Data Gallery

  • p300 antibody (mAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with chromatin from 25 ug LNCaP cel extract and 5 ug of p300 antibody. ChIP DNA was sequenced on the Illumina HiSeq and 6 million sequence tags were mapped to identify p300 binding sites on chromosome 7.

    p300 antibody (mAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with chromatin from 25 ug LNCaP cel extract and 5 ug of p300 antibody. ChIP DNA was sequenced on the Illumina HiSeq and 6 million sequence tags were mapped to identify p300 binding sites on chromosome 7.

  • p300 antibody (mAb) tested by ChIP. ChIP was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with chromatin from 4.5 million LNCaP cells and 4 ug of p300 antibody. ChIP DNA was used in qPCR with the negative control primer pair or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Active Motif's Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.

    p300 antibody (mAb) tested by ChIP. ChIP was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with chromatin from 4.5 million LNCaP cells and 4 ug of p300 antibody. ChIP DNA was used in qPCR with the negative control primer pair or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Active Motif's Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.

  • p300 antibody (mAb) tested by immunofluorescence. Formaldehyde fixed HeLa cells stained with p300 antibody at a 0.5 ug/ml dilution.

    p300 antibody (mAb) tested by immunofluorescence. Formaldehyde fixed HeLa cells stained with p300 antibody at a 0.5 ug/ml dilution.

Product Information

Tested Applications ChIP, ChIP-Seq, ICC, IF, IP, WB

Applications Validated by Active Motif: ChIP: 5 ug per ChIP ChIP-Seq: 5 ug each ICC/IF: 0.5 ug/ml dilution
Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen The antibody was raised against full-length p300 purified from human 293 cells.
Full Name p300 antibody (mAb) (Clone NM11)
Synonyms p300, E1A binding protein p300, RSTS2, KAT3B, E1A-binding protein, 300kD, histone acetyltransferase p300, E1A-associated protein p300, P300, p300 HAT, antibody, monoclonal, mAb, immunoprecipitation, ip, immunofluorescence, if, western blotting, wb, sample
Molecular weight 300 kDa
GenBank accession numberNP_001420
RRIDAB_2716754
Purification Method Protein A Chromatography
Buffer Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

p300 (E1A binding protein p300) functions as a histone acetyltransferase and regulates transcription via chromatin remodeling. Acetylates all four core histones in nucleosomes. Histone acetylation gives an epigenetic tag for transcriptional activation. Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. Also functions as an acetyltransferase for nonhistone targets. Acetylates 'Lys-131' of ALX1 and acts as its coactivator in the presence of CREBBP. Acetylates SIRT2 and is proposed to indirectly increase the transcriptional activity of TP53 through acetylation and subsequent attenuation of SIRT2 deacetylase function. Acetylates HDAC1 leading to its inactivation and modulation of transcription. Can also mediate transcriptional repression. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein.