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alpha Tubulin antibody (mAb) (Clone 5-B-1-2)

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Wide Range Predicted

Applications

ICC, IF, WB

CloneNo.

5-B-1-2

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Cat No : 39527,39528 39527

Synonyms

a-tubulin, alpha tubulin, tubulin, clone 5-B-1-2, mAb, monoclonal, K-alpha-1, antibody, antibodies, sample

Validation Data Gallery

  • alpha Tubulin mAb (Clone 5-B-1-2) tested by immunofluorescence. Top: HeLa cells stained with alpha Tubulin mAb at 1:500 dilution (red). Middle: Same cells stained with DAPI (blue). Bottom: Both images merged. Staining was carried out using MAX Stain Immunofluorescence Tools.

    alpha Tubulin mAb (Clone 5-B-1-2) tested by immunofluorescence. Top: HeLa cells stained with alpha Tubulin mAb at 1:500 dilution (red). Middle: Same cells stained with DAPI (blue). Bottom: Both images merged. Staining was carried out using MAX Stain Immunofluorescence Tools.

  • alpha Tubulin mAb (Clone 5-B-1-2) tested by Western blot. HeLa whole-cell extract (15 ug/lane) probed with alpha Tubulin mAb (1:5,000 dilution).

    alpha Tubulin mAb (Clone 5-B-1-2) tested by Western blot. HeLa whole-cell extract (15 ug/lane) probed with alpha Tubulin mAb (1:5,000 dilution).

Product Information

Tested Applications ICC, IF, WB

Applications Validated by Active Motif: WB*: 1:500 - 1:2,500 dilution ICC/IF: 1:1,000 - 1:10,000 dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.
Tested Reactivity Human, Mouse, Wide Range Predicted
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen This alpha Tubulin antibody was raised against total protein purified from sea urchin sperm axonemes.
Full Name alpha Tubulin antibody (mAb) (Clone 5-B-1-2)
Synonyms a-tubulin, alpha tubulin, tubulin, clone 5-B-1-2, mAb, monoclonal, K-alpha-1, antibody, antibodies, sample
Molecular weight 52 kDa
GenBank accession numberN/A
RRIDAB_2793243
Purification Method Ascites
Buffer Ascites containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of a-tubulin and b-tubulin. Alpha Tubulin expression is reduced in cells deficient in MeCP2, leading to conditions such as Rett syndrome and autistic-spectrum disorders.