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XRCC1 antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

ICC, IF, WB

Activ Motif logo

Cat No : 61371,61372 61371

Synonyms

XRCC1, X-ray repair complementing defective repair in Chinese hamster cells 1, RCC, X-ray repair cross-complementing protein 1, DNA repair protein XRCC1, X-ray-repair, complementing defective, repair in Chinese hamster, antibody, polyclonal, western blot, pAb, sample

Validation Data Gallery

  • XRCC1 antibody (pAb) tested by Immunofluorescence. Formaldehyde fixed HeLa cells stained with XRCC1 antibody at a 1:2,000 dilution.

    XRCC1 antibody (pAb) tested by Immunofluorescence. Formaldehyde fixed HeLa cells stained with XRCC1 antibody at a 1:2,000 dilution.

  • XRCC1 antibody (pAb) tested by Western blot. XRCC1 detection by Western blot. The analysis was performed using 30 ug HeLa nuclear cell extract and XRCC1 at a 1:500 dilution.

    XRCC1 antibody (pAb) tested by Western blot. XRCC1 detection by Western blot. The analysis was performed using 30 ug HeLa nuclear cell extract and XRCC1 at a 1:500 dilution.

Product Information

Tested Applications ICC, IF, WB

Applications Validated by Active Motif: ICC/IF: 1:2,000 dilution WB*: 1:500 - 1:1,000 dilution The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required. *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.
Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen This antibody was raised against a recombinant protein corresponding to the N-terminal region of human XRCC1.
Full Name XRCC1 antibody (pAb)
Synonyms XRCC1, X-ray repair complementing defective repair in Chinese hamster cells 1, RCC, X-ray repair cross-complementing protein 1, DNA repair protein XRCC1, X-ray-repair, complementing defective, repair in Chinese hamster, antibody, polyclonal, western blot, pAb, sample
Molecular weight 85 kDa
GenBank accession numberNP_006288
RRIDAB_2793610
Purification Method Affinity Purified
Buffer Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

XRCC1 (X-ray repair complementing defective repair in Chinese hamster cells 1) corrects defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. XRCC1 is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. This protein interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase (PARP) to participate in the base excision repair (BER) pathway.