Uhrf1 antibody (mAb) (Clone 1RC-1C10)

Host / Isotype

Mouse / IgG1, k

Reactivity

Human

Applications

ICC, IF, IHC, IP, WB

CloneNo.

1RC-1C10

Cat No : 61341

Synonyms

Uhrf1, Uhrf 1, ICBP90, RNF106, NP95, transcription factor, ubiquitin-like, polyclonal, antibody, antibodies

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Validation Data Gallery

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  Uhrf1 antibody (mAb) tested by immunofluorescence. Formaldehyde fixed HeLa cells stained with Uhrf1 antibody at a 0.5 ug/ml dilution.

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  Uhrf1 antibody (mAb) tested by Western blot. 20 ug of HeLa nuclear extract was run on SDS-PAGE and probed with antibody at 1:1000 dilution.

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Product Information

• Tested Applications: ICC, IF, IHC, IP, WB

  Applications Validated by Active Motif: ICC/IF: 0.5 ug/ml dilution WB*: 1:1000 - 1:2,500 dilution *Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot. In addition, we recommend the addition of 0.05% Tween 20 to all blocking solutions to reduce background.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1, k
• Class: Monoclonal
• Type: Antibody
• Immunogen: This antibody was raised against a synthetic peptide corresponding to amino acid residue(s) 263-793 of human Uhrf1.
• Full Name: Uhrf1 antibody (mAb) (Clone 1RC-1C10)
• Synonyms: Uhrf1, Uhrf 1, ICBP90, RNF106, NP95, transcription factor, ubiquitin-like, polyclonal, antibody, antibodies
• Molecular weight: 96 kDa
• GenBank accession number: NP_001041666
• Purification Method: Protein G Chromatography
• Buffer: Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide. Sodium azide is highly toxic.
• Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

Uhrf1 (ubiquitin-like, containing PHD and RING finger domains 1) is a transcription and cell cycle regulator. It functions as a putative E3 ubiquitin-protein ligase. Uhrf1 is important for G1/S transition of the cell cycle. It is also believed that Uhrf1 may help recruit the DNA methyltransferase, DNMT1, to hemimethylated DNA and assist in the stabilization of DNMT1 with chromatin.