Tet3 antibody (mAb) (Clone 23B9)
Host / Isotype
Rat / IgG2a
Reactivity
Mouse
Applications
ICC, IF, IP, WB
CloneNo.
23B9
Cat No : 61743,61744 61743
Synonyms
Validation Data Gallery
Product Information
| Tested Applications | ICC, IF, IP, WB |
| Tested Reactivity | Mouse |
| Host / Isotype | Rat / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | This antibody was raised against a recombinant protein corresponding amino acids 976-1521 of mouse Tet3. |
| Full Name | Tet3 antibody (mAb) (Clone 23B9) |
| Synonyms | Tet Methylcytosine Dioxygenase 3, Tet Oncogene Family Member 3, EC 1.14.11.n2, KIAA0401, Probable Methylcytosine Dioxygenase TET3, Putative Methylcytosine Dioxygenase, Methylcytosine Dioxygenase TET3, HCG_40738, antibody, antibodies, monoclonal, mab, if, icc, ip, wb, immunofluorescence, immunocytochemistry, immunoprecipitation, western blotting, sample |
| Molecular weight | 180 kDa |
| GenBank accession number | NP_898961 |
| RRID | AB_2793753 |
| Purification Method | Protein G Chromatography |
| Buffer | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| Storage | Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage. |
Background Information
Tet3 (Tet Methylcytosine Dioxygenase 3) is a dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in epigenetic chromatin reprogramming in the zygote following fertilization. Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in DNA demethylation in the paternal pronucleus by mediating conversion of 5mC into 5hmC, 5fC and 5caC. Does not mediate DNA demethylation of maternal pronucleus because of the presence of DPPA3/PGC7 on maternal chromatin that prevents TET3-binding to chromatin (By similarity). In addition to its role in DNA demethylation, also involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT.