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TRAF2 antibody (mAb) (Clone 33A1293)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB

CloneNo.

33A1293

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Cat No : 40919 40919

Synonyms

TRAF2, TRAF-2, TRAF 2, mAb, monoclonal, TNF receptor-associated factor 2, TRAP, antibody, antibodies

Validation Data Gallery

  • TRAF2 mAb tested by Western blot. TRAF2 detection by Western blot. The analysis was performed using 30 ug HeLa nuclear extract and TRAF2 mAb at a 2 ug/ml dilution.

    TRAF2 mAb tested by Western blot. TRAF2 detection by Western blot. The analysis was performed using 30 ug HeLa nuclear extract and TRAF2 mAb at a 2 ug/ml dilution.

Product Information

Tested Applications WB

Applications Validated by Active Motif: WB: 1 - 2 ug/ml dilution For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required.
Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen This TRAF2 antibody was raised against a fusion protein corresponding to amino acid residues 215 to 222 of human TRAF2.
Full Name TRAF2 antibody (mAb) (Clone 33A1293)
Synonyms TRAF2, TRAF-2, TRAF 2, mAb, monoclonal, TNF receptor-associated factor 2, TRAP, antibody, antibodies
Molecular weight 57 kDa
GenBank accession numberNP_066961
RRIDAB_2793438
Purification Method Affinity Purified
Buffer PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

TRAF2 (TNF receptor-associated factor 2, TRAP) directly interacts with TNF receptors, and forms a heterodimeric complex with TRAF1. This protein is required for TNF-alpha-mediated activation of MAPK8/JNK and NFkB (NFkB p50 & NFkB p65). The protein complex formed by TRAF2 and TRAF1 interact with the inhibitor-of-apoptosis proteins (Livin/ML-IAP for example), and function as a mediator of the anti-apoptotic signals from TNF receptors. TRAF2 interaction with TRADD, a TNF receptor associated apoptotic signal transducer, ensures the recruitment of IAPs for the direct inhibition of caspase activation (Caspase-3, Caspase-7, Caspase-8, Caspase-9 and Caspase-14). BIRC2/c-IAP1, an apoptosis inhibitor possessing ubiquitin ligase activity, can unbiquitinate and induce the degradation of this protein, and thus potentiate TNF-induced apoptosis.