Sox2 antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

ChIP, ChIP-Seq, ICC, IF, WB

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Cat No : 39843,39844 39843

Synonyms

Sox2, SRY related HMG BOX gene 2, sox, sox 2, sox-2, embryogenesis, embryonic stem cell pluripotency, es cell, esc, Induced Pluripotency, microphthalmia, sample



Product Information

Tested Applications ChIP, ChIP-Seq, ICC, IF, WB

Applications Validated by Active Motif: ChIP: 10 ul per ChIP ChIP-Seq: 10 ul each ICC/IF: 1:200 dilution WB: 1:500 - 1:1,000 dilution ChIP-Seq validation was performed by Active Motif's Epigenetics Services; the complete data set is available in the UCSC Genome Browser by clicking here.

Tested Reactivity Human, Mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen This Sox2 antibody was raised against a peptide within the C-terminal region of human Sox2.
Full Name Sox2 antibody (pAb)
Synonyms Sox2, SRY related HMG BOX gene 2, sox, sox 2, sox-2, embryogenesis, embryonic stem cell pluripotency, es cell, esc, Induced Pluripotency, microphthalmia, sample
Molecular weight 42 kDa
GenBank accession numberNP_003097
RRIDAB_2750954
Purification Method Affinity Purified
Buffer Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

Sox2 (SRY related HMG BOX gene 2) is a DNA binding transcription factor and a member of the SOX family of proteins. SOX proteins have an HMG box that binds DNA. Sox2 forms a complex with Oct-4 and controls the expression of a number of genes involved in embryonic. Sox2 is critical for early embryogenesis and for embryonic stem cell pluripotency and thus can serve as a stem cell marker. Overexpression of Sox2 (along with Oct-4, KLF4 and c-Myc) can transform mouse fibroblasts into a state resembling embryonic stem cells (ES cells), referred to as Induced Pluripotency. Defects in Sox2 are the cause of microphthalmia syndromic type 3.