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SMC4 antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IP, WB

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Cat No : 61129,61130 61129

Synonyms

Structural Maintenance of Chromosomes 4, smc4, smc-4, mitotic chromosome organization, condensing, mitosis, smc2, ATPase, smc, western blotting, wb, immunopreciptiation, ip, polyclonal, pab, antibody, sample

Validation Data Gallery

  • SMC4 antibody (pAb) tested by Immunoprecipitation. 10 ul of SMC4 antibody was used to immunoprecipitate SMC4 from 500 ug of HeLa nuclear cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the SMC4 antibody at a dilution of 1:1,000.

    SMC4 antibody (pAb) tested by Immunoprecipitation. 10 ul of SMC4 antibody was used to immunoprecipitate SMC4 from 500 ug of HeLa nuclear cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the SMC4 antibody at a dilution of 1:1,000.

  • SMC4 antibody (pAb) tested by Western blot Nuclear extract of HeLa cells (20 μg) probed with SMC4 antibody (1:1,000).

    SMC4 antibody (pAb) tested by Western blot Nuclear extract of HeLa cells (20 μg) probed with SMC4 antibody (1:1,000).

Product Information

Tested Applications IP, WB

Applications Validated by Active Motif: IP: 10 ul per IP WB*: 1:500 - 1:1,000 dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.
Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen This SMC4 antibody was raised against a peptide from the N-terminus of human SMC4.
Full Name SMC4 antibody (pAb)
Synonyms Structural Maintenance of Chromosomes 4, smc4, smc-4, mitotic chromosome organization, condensing, mitosis, smc2, ATPase, smc, western blotting, wb, immunopreciptiation, ip, polyclonal, pab, antibody, sample
Molecular weight 175 kDa
GenBank accession numberNP_001002800
RRIDAB_2793519
Purification Method Affinity Purified
Buffer Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

The Structural Maintenance of Chromosomes (SMC) family proteins play critical roles in various nuclear events that require structural changes of chromosomes, including mitotic chromosome organization, DNA recombination and repair and global transcriptional repression. SMC4 is the central component of the condensin complex, a complex required for conversion of interphase chromatin into condensed mitotic chromosomes. SMC4 forms a heterodimer with SMC2 and contains a myosin-like ATPase domain that serves as a molecular motor to help organize chromatin. During mitosis, most of the condensin complex is associated with the chromatin.