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SMARCC1 / BAF155 antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

ICC, IF, WB

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Cat No : 61409,61410 61409

Synonyms

SMARCC1 / BAF155, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 1, SWI/SNF complex 155 kDa subunit, BAF155, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1, CRACC1, SWI3, Rsc8, chromatin remodeling complex BAF155 subunit, SRG3, mammalian chromatin remodeling complex BRG1-associated factor 155, BRG1-associated factor 155, SWI/SNF complex subunit SMARCC1, antibody, polyclonal, pAb, western blotting, wb, sample

Validation Data Gallery

  • SMARCC1 / BAF155 antibody (pAb) tested by Immunofluorescence. Formaldehyde fixed HeLa cells stained with SMARCC1 / BAF155 antibody at a 1:2,000 dilution.

    SMARCC1 / BAF155 antibody (pAb) tested by Immunofluorescence. Formaldehyde fixed HeLa cells stained with SMARCC1 / BAF155 antibody at a 1:2,000 dilution.

  • SMARCC1 / BAF155 antibody (pAb) tested by Western blot. K-562 nuclear extract (30 ug) probed with SMARCC1 / BAF155 antibody at a dilution of 1:500.

    SMARCC1 / BAF155 antibody (pAb) tested by Western blot. K-562 nuclear extract (30 ug) probed with SMARCC1 / BAF155 antibody at a dilution of 1:500.

Product Information

Tested Applications ICC, IF, WB

Applications Validated by Active Motif: ICC/IF: 1:2,000 dilution WB*: 1:500 - 1:1,000 dilution The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required. *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.
Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen This antibody was raised against a peptide within the N-terminal region of human SMARCC1 / BAF155.
Full Name SMARCC1 / BAF155 antibody (pAb)
Synonyms SMARCC1 / BAF155, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 1, SWI/SNF complex 155 kDa subunit, BAF155, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1, CRACC1, SWI3, Rsc8, chromatin remodeling complex BAF155 subunit, SRG3, mammalian chromatin remodeling complex BRG1-associated factor 155, BRG1-associated factor 155, SWI/SNF complex subunit SMARCC1, antibody, polyclonal, pAb, western blotting, wb, sample
Molecular weight 155 kDa
GenBank accession numberNP_003065
RRIDAB_2793624
Purification Method Affinity Purified
Buffer Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

SMARCC1 / BAF155 (SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 1 / BRG1-associated factor 155) is involved in transcriptional activation and repression of select genes by chromatin remodeling. Component of multiple SWI/SNF chromatin remodeling complexes including Swi/Snf-A (BAF), Swi/Snf-B (PBAF), Brm, Brg1(I), WINAC, Brg1(II), npBAF and nBAF.