SMARCB1 antibody (mAb) (Clone 2C2)
Host / Isotype
Mouse / IgG2a
Reactivity
Human
Applications
ICC, IF, WB
CloneNo.
2C2
Cat No : 61649,61650 61649
Synonyms
Validation Data Gallery
Product Information
| Tested Applications |
ICC, IF, WB
Applications Validated by Active Motif: ICC/IF: 2 - 10 ug/ml dilution WB*: 0.5 - 2 ug/ml dilution The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required. *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot. |
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | This antibody was raised against full-length recombinant human SMARCB1 protein. |
| Full Name | SMARCB1 antibody (mAb) (Clone 2C2) |
| Synonyms | SMARCB1, SWI/SNF Related, Matrix Associated Actin Dependent Regulator Of Chromatin, Subfamily B Member 1, SNF5, SNF5L1, SWNTS1, INI1, Homolog-Like 1, Malignant Rhabdoid Tumor Suppressor, Integrase Interactor 1, Integrase Interactor 1 Protein, Sucrose Nonfermenting, BAF47, yeast, BRG1-Associated Factor 47, Sfh1p, hSNF5, Snr1, SNF5 Homolog, hSNFS, MRD15, Sucrose Nonfermenting Yeast, Homolog-Like 1, RDT, SWI/SNF-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin Subfamily B Member 1, RTPS1, SWI/SNF-Related Matrix-Associated Protein, monoclonal, mab, immunofluorescence, if, immunocytochemistry, icc, western blotting, wb, antibody, antibodies, sample |
| Molecular weight | 45 kDa |
| GenBank accession number | NP_001007469 |
| RRID | AB_2793719 |
| Purification Method | Protein A Chromatography |
| Buffer | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| Storage | Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage. |
Background Information
SMARCB1 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator Of Chromatin, Subfamily B, Member 1) is a core component of the BAF (hSWI/SNF) complex. This ATP-dependent chromatin-remodeling complex plays important roles in cell proliferation and differentiation, in cellular antiviral activities and inhibition of tumor formation. The BAF complex is able to create a stable, altered form of chromatin that constrains fewer negative supercoils than normal. This change in supercoiling would be due to the conversion of up to one-half of the nucleosomes on polynucleosomal arrays into asymmetric structures, termed altosomes, each composed of 2 histones octamers. Stimulates in vitro the remodeling activity of SMARCA4/BRG1/BAF190A. Involved in activation of CSF1 promoter. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Plays a key role in cell-cycle control and causes cell cycle arrest in G0/G1. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.