SMARCAL1 / HARP antibody (mAb) (Clone 4B10)
Host / Isotype
Mouse / IgG1
Reactivity
Bovine, Human, Mouse
Applications
IP, WB
CloneNo.
4B10
Cat No : 61115,61116 61115
Synonyms
Validation Data Gallery
Product Information
Tested Applications | IP, WB |
Tested Reactivity | Bovine, Human, Mouse |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | This SMARCAL1 / HARP antibody was raised against purifed bovine SMARCAL1 / HARP protein. |
Full Name | SMARCAL1 / HARP antibody (mAb) (Clone 4B10) |
Synonyms | SMARCAL1, HARP, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1, HepA-related protein, DNA-dependent ATPase A, SWI / SNF, ATP-dependent annealing helicase activity, DNA replication forks, Replication Protein A, RPA, Schimke immuno-osseous dysplasia, SIOD, SIOD causes spondyloepiphyseal dysplasia, bone growth, short stature, skeletal abnormalities, problems with vision and hearing, renal dysfunction and T-cell immunodeficiency, sample, polyclonal |
Molecular weight | 150 kDa |
GenBank accession number | NP_788839 |
RRID | AB_2614999 |
Purification Method | Protein G Chromatography |
Buffer | Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
Storage | Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage. |
Background Information
SMARCAL1 / HARP (SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1, HepA-related protein, DNA-dependent ATPase A) is a member of the SWI / SNF family of ATPases and chromatin remodeling proteins. Unlike other SWI / SNF family members, SMARCAL1 / HARP has not been shown to remodel chromatin, but possesses an ATP-dependent annealing helicase activity. SMARCAL1 reanneals single-stranded DNA bubbles in DNA at DNA replication forks that are bound by Replication Protein A (RPA). Defects in SMARCAL1 / HARP are a cause of Schimke immuno-osseous dysplasia (SIOD) SIOD causes spondyloepiphyseal dysplasia (a disorder of bone growth that results in short stature, skeletal abnormalities, and problems with vision and hearing), renal dysfunction and T-cell immunodeficiency.