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SMARCA4 antibody (mAb) (Clone 5B7)

Host / Isotype

Rat / IgG2a

Reactivity

Human, Monkey, Mouse

Applications

ICC, IF, WB

CloneNo.

5B7

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Cat No : 61625,61626 61625

Synonyms

SMARCA4, SWI/SNF Related Matrix Associated Actin Dependent Regulator Of Chromatin Subfamily A Member 4, SNF2-beta, SNF2L4, BRG1-Associated Factor 190A, ATP-Dependent Helicase SMARCA4, MRD16, Mitotic Growth And Transcription Activator, RTPS2, BRG1, BAF190, Brahma Protein-Like 1, SNF2, BRM/SWI2-Related Gene 1, SNF2LB, Global Transcription Activator Homologous Sequence, SWI2, Homeotic Gene Regulator, hSNF2b, Nuclear Protein GRB1, Transcription Activator BRG1, SNF2-Like 4, EC 3.6.4., Sucrose Nonfermenting-Like 4, SNF2B, Protein Brahma Homolog 1, SWI/SNF-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin Subfamily A Member 4, Protein BRG-1, EC 3.6.1, BAF190A, transcription, coactivator, activation, monoclonal, mab, western blotting, wb, immunocytochemistry, icc, immunofluorescence, if, antibody, antibodies, sample

Validation Data Gallery

  • SMARCA4 antibody (mAb) (Clone 5B7) tested by immunofluorescence. Top: HeLa cells stained with SMARCA4 antibody (mAb). Bottom: Hoechst staining.

    SMARCA4 antibody (mAb) (Clone 5B7) tested by immunofluorescence. Top: HeLa cells stained with SMARCA4 antibody (mAb). Bottom: Hoechst staining.

  • SMARCA4 antibody (mAb) (Clone 5B7) tested by Western blot. Whole cell extracts were probed with SMARCA4 antibody (mAb). Lane 1: L929 cells. Lane 2: HeLa cells. Lane 3: Cos cells

    SMARCA4 antibody (mAb) (Clone 5B7) tested by Western blot. Whole cell extracts were probed with SMARCA4 antibody (mAb). Lane 1: L929 cells. Lane 2: HeLa cells. Lane 3: Cos cells

Product Information

Tested Applications ICC, IF, WB

Applications Validated by Active Motif: ICC/IF: 2 - 10 ug/ml dilution WB: 0.5 - 2 ug/ml dilution
Tested Reactivity Human, Monkey, Mouse
Host / Isotype Rat / IgG2a
Class Monoclonal
Type Antibody
Immunogen This antibody was raised against a recombinant protein corresponding to amino acids 213-295 of human SMARCA4.
Full Name SMARCA4 antibody (mAb) (Clone 5B7)
Synonyms SMARCA4, SWI/SNF Related Matrix Associated Actin Dependent Regulator Of Chromatin Subfamily A Member 4, SNF2-beta, SNF2L4, BRG1-Associated Factor 190A, ATP-Dependent Helicase SMARCA4, MRD16, Mitotic Growth And Transcription Activator, RTPS2, BRG1, BAF190, Brahma Protein-Like 1, SNF2, BRM/SWI2-Related Gene 1, SNF2LB, Global Transcription Activator Homologous Sequence, SWI2, Homeotic Gene Regulator, hSNF2b, Nuclear Protein GRB1, Transcription Activator BRG1, SNF2-Like 4, EC 3.6.4., Sucrose Nonfermenting-Like 4, SNF2B, Protein Brahma Homolog 1, SWI/SNF-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin Subfamily A Member 4, Protein BRG-1, EC 3.6.1, BAF190A, transcription, coactivator, activation, monoclonal, mab, western blotting, wb, immunocytochemistry, icc, immunofluorescence, if, antibody, antibodies, sample
Molecular weight 180 kDa
GenBank accession numberNP_001122321
RRIDAB_2793708
Purification Method Protein G Chromatography
Buffer Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

SMARCA4 (SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4) is the central catalytic ATPase subunit of numerous chromatin-remodeling complexes, including SWI/SNF. These complexes are able to use energy from ATP hydrolysis to physically disrupt chromatin architecture of target promoters and facilitate the binding of transcription factors to nucleosomal DNA. SMARCA4 is a co-regulator of transcription and has been implicated in the activation and repression of gene expression through the modulation of chromatin in various tissues and physiological conditions. This protein plays a major role in many cellular processes such as DNA replication, repair and recombination. Mammalian SMARCA4 is usually associated with approximately 10-12 BAF subunits or other proteins involved in regulation of gene expression. SMARCA4 is part of the stem cell-specific BAF complex, esBAF, and is involved in regulating stem cell pluripotency.