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SMARCA2 / BRM antibody (mAb) (Clone 1H7A10)

Host / Isotype

Rat / IgG2a

Reactivity

Human, Mouse

Applications

ChIP, ICC, IF, WB

CloneNo.

1H7A10

Activ Motif logo

Cat No : 39805,39806 39805

Synonyms

SMARCA2 / BRM, BRM, Brahma, SMARCA2, SNF2L2, SWI/SNF, ATP-dependant chromatin remodeling, antibody, antibodies, clone 1H7A10, sample, snf2a, snf2, trithorax, trxg, trx

Validation Data Gallery

  • SMARCA2 / BRM antibody (mAb) tested by ChIP. Chromatin IP performed using the ChIP-IT Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 10 ug of SMARCA2 / BRM antibody or the equivalent amount of rat IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the CD44 gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.

    SMARCA2 / BRM antibody (mAb) tested by ChIP. Chromatin IP performed using the ChIP-IT Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 10 ug of SMARCA2 / BRM antibody or the equivalent amount of rat IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the CD44 gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.

  • SMARCA2 / BRM antibody (mAb) tested by Immunofluorescence. Formaldehyde fixed HeLa cells stained with SMARCA2 / BRM antibody at a 0.5 ug/ml dilution.

    SMARCA2 / BRM antibody (mAb) tested by Immunofluorescence. Formaldehyde fixed HeLa cells stained with SMARCA2 / BRM antibody at a 0.5 ug/ml dilution.

Product Information

Tested Applications ChIP, ICC, IF, WB

Applications Validated by Active Motif: ChIP: 10 ug per ChIP ICC/IF: 0.5 ug/ml dilution
Tested Reactivity Human, Mouse
Host / Isotype Rat / IgG2a
Class Monoclonal
Type Antibody
Immunogen This SMARCA2 / BRM antibody was raised against a recombinant protein corresponding to amino acids 48-214 of mouse BRM.
Full Name SMARCA2 / BRM antibody (mAb) (Clone 1H7A10)
Synonyms SMARCA2 / BRM, BRM, Brahma, SMARCA2, SNF2L2, SWI/SNF, ATP-dependant chromatin remodeling, antibody, antibodies, clone 1H7A10, sample, snf2a, snf2, trithorax, trxg, trx
Molecular weight 190 kDa
GenBank accession numberNP_035546
RRIDAB_2615072
Purification Method Protein G Chromatography
Buffer Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2 (SMARCA2), also known as BRM, is a member of the SWI/SNF family of ATP-dependant chromatin remodeling proteins. SMARCA2 is the catalytic subunit of a multi-subunit protein complex that utilizes the energy from ATP hydrolysis to alter chromatin structure and activate transcription.