SMAD3 antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB

Cat No : 61249,61250

Synonyms

SMAD3, mothers against decapentaplegic homolog 3, mad3, MAD homolog 3, mothers against DPP homolog 3, hSMAD3, SMAD family member 3, drosophila, c.elegans, transcription modulator, antibody, polyclonal, sample, western blot, stem cell, stem cells

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Validation Data Gallery

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  SMAD3 antibody (pAb) tested by Western blot. Whole cell extract of A549 cells (30 ug) probed with SMAD3 antibody (1:500).

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Product Information

• Tested Applications: WB

  Applications Validated by Active Motif: WB: 1:500 - 1:1,000 dilution The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: This SMAD3 antibody was raised against a peptide within human SMAD3 (mothers against decapentaplegic homolog 3).
• Full Name: SMAD3 antibody (pAb)
• Synonyms: SMAD3, mothers against decapentaplegic homolog 3, mad3, MAD homolog 3, mothers against DPP homolog 3, hSMAD3, SMAD family member 3, drosophila, c.elegans, transcription modulator, antibody, polyclonal, sample, western blot, stem cell, stem cells
• Molecular weight: 52 kDa
• GenBank accession number: NP_005893
• RRID: AB_2793568
• Purification Method: Affinity Purified
• Buffer: Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
• Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

This SMAD3 (mothers against decapentaplegic homolog 3, mad3, MAD homolog 3) protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.