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SIN3A antibody (pAb)

Host / Isotype

Rabbit / Serum

Reactivity

Human, Mouse

Applications

ChIP, WB

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Cat No : 39865,39866 39865

Synonyms

SIN3A, Switch independent 3 homologue, co-repressor, REST, MXI1, MAD-MAX, HDAC1, HDAC2, SMRT, NCoR1, sample, sin3, sin 3a, sin3 a, stem cell, stem cells, acetylation, deacetylation, chiphsabs

Validation Data Gallery

  • SIN3A antibody (pAb) tested by ChIP. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 30 ug of chromatin from TM4 Sertoli cells and 5 ul of SIN3A antibody. ChIP DNA was used in qPCR with the control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Active Motif's Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.

    SIN3A antibody (pAb) tested by ChIP. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 30 ug of chromatin from TM4 Sertoli cells and 5 ul of SIN3A antibody. ChIP DNA was used in qPCR with the control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Active Motif's Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.

  • SIN3A antibody (pAb) tested by Western blot. Nuclear extract of HeLa cells (20 μg) probed with SIN3A antibody at a dilution of 1:5,000.

    SIN3A antibody (pAb) tested by Western blot. Nuclear extract of HeLa cells (20 μg) probed with SIN3A antibody at a dilution of 1:5,000.

Product Information

Tested Applications ChIP, WB

Applications Validated by Active Motif: ChIP: 5 - 10 ul per ChIP WB*: 1:1,000 - 1:5,000 dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.
Tested Reactivity Human, Mouse
Host / Isotype Rabbit / Serum
Class Polyclonal
Type Antibody
Immunogen This SIN3A antibody was raised against a peptide derived from the N-terminus of human SIN3A.
Full Name SIN3A antibody (pAb)
Synonyms SIN3A, Switch independent 3 homologue, co-repressor, REST, MXI1, MAD-MAX, HDAC1, HDAC2, SMRT, NCoR1, sample, sin3, sin 3a, sin3 a, stem cell, stem cells, acetylation, deacetylation, chiphsabs
Molecular weight 155 kDa
GenBank accession numberNP_001138829
RRIDAB_2793373
Purification Method None
Buffer Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

SIN3A (Switch independent 3 homologue) is the mammalian homologue of the yeast protein Sin3. SIN3A is transcriptional co-repressor that is recruited to promoters by sequence-specific DNA binding proteins, such as REST, MXI1 and MAD-MAX. It is part of a large multi-subunit repressor protein complex that also contains HDAC1, HDAC2 and SMRT/NCoR1.