SIN3A antibody (pAb)

Host / Isotype

Rabbit / Serum

Reactivity

Human, Mouse

Applications

ChIP, WB

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Cat No : 39865,39866 39865

Synonyms

SIN3A, Switch independent 3 homologue, co-repressor, REST, MXI1, MAD-MAX, HDAC1, HDAC2, SMRT, NCoR1, sample, sin3, sin 3a, sin3 a, stem cell, stem cells, acetylation, deacetylation, chiphsabs



Product Information

Tested Applications ChIP, WB

Applications Validated by Active Motif: ChIP: 5 - 10 ul per ChIP WB*: 1:1,000 - 1:5,000 dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

Tested Reactivity Human, Mouse
Host / Isotype Rabbit / Serum
Class Polyclonal
Type Antibody
Immunogen This SIN3A antibody was raised against a peptide derived from the N-terminus of human SIN3A.
Full Name SIN3A antibody (pAb)
Synonyms SIN3A, Switch independent 3 homologue, co-repressor, REST, MXI1, MAD-MAX, HDAC1, HDAC2, SMRT, NCoR1, sample, sin3, sin 3a, sin3 a, stem cell, stem cells, acetylation, deacetylation, chiphsabs
Molecular weight 155 kDa
GenBank accession numberNP_001138829
RRIDAB_2793373
Purification Method None
Buffer Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

SIN3A (Switch independent 3 homologue) is the mammalian homologue of the yeast protein Sin3. SIN3A is transcriptional co-repressor that is recruited to promoters by sequence-specific DNA binding proteins, such as REST, MXI1 and MAD-MAX. It is part of a large multi-subunit repressor protein complex that also contains HDAC1, HDAC2 and SMRT/NCoR1.