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Ring1B antibody (mAb) (Clone 3-3)

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Monkey, Mouse

Applications

ChIP, ChIP-Seq, ICC, IF, IP, WB

CloneNo.

3-3

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Cat No : 39063,39663,39664 39663

Synonyms

Ring1B, Ring 1B, ring finger protein, Ring2, RNF2, polycomb group, PcG, PRC1, transcription, development, E3 ubiquitin ligase, mouse, monoclonal, Ab, antibody, antibodies, chip, chromatin immunoprecipitation, stem, sample, stem cell, stem cells, chiphsabs

Validation Data Gallery

  • Ring1B antibody (mAb) tested by ChIP-chip. ChIP was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with chromatin from 4.5 million human embryonic stem cells and 4 ul of antibody. ChIP DNA was amplified by WGA, labeled and hybridized to a human tiling array. The two images show regions of strong binding on chromosomes 2 and 9.

    Ring1B antibody (mAb) tested by ChIP-chip. ChIP was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with chromatin from 4.5 million human embryonic stem cells and 4 ul of antibody. ChIP DNA was amplified by WGA, labeled and hybridized to a human tiling array. The two images show regions of strong binding on chromosomes 2 and 9.

  • Ring1B antibody (mAb) tested by ChIP-Seq. ChIP was performed using Ring1B antibody with 30 ug chromatin from the wild-type mouse ESC and 4 ug of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 19.5 million sequence tags were mapped to identify Ring1B binding sites. The image shows binding across a region of chromosome 2.

    Ring1B antibody (mAb) tested by ChIP-Seq. ChIP was performed using Ring1B antibody with 30 ug chromatin from the wild-type mouse ESC and 4 ug of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 19.5 million sequence tags were mapped to identify Ring1B binding sites. The image shows binding across a region of chromosome 2.

Product Information

Tested Applications ChIP, ChIP-Seq, ICC, IF, IP, WB

Applications Validated by Active Motif: ChIP-Seq: 4 ul each
Tested Reactivity Human, Monkey, Mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen This Ring1B antibody was raised against the recombinant full-length mouse protein.
Full Name Ring1B antibody (mAb) (Clone 3-3)
Synonyms Ring1B, Ring 1B, ring finger protein, Ring2, RNF2, polycomb group, PcG, PRC1, transcription, development, E3 ubiquitin ligase, mouse, monoclonal, Ab, antibody, antibodies, chip, chromatin immunoprecipitation, stem, sample, stem cell, stem cells, chiphsabs
Molecular weight 40 kDa
GenBank accession numberNP_009143
RRIDAB_2615006
Purification Method Protein G Chromatography
Buffer Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

Ring1B (also known as ring finger protein 2, RING2 or RNF2) is an essential component of the Polycomb group (PcG) multiprotein PRC1 complex. The PRC1 complex is important for the transcriptional repression of genes involved in development and cell proliferation, such as the Hox genes. Ring1B also acts as an E3 ubiquitin ligase on histone H2A of the PRC1 complex.