• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Recombinant YY1 protein

Expressed In

Baculovirus

Protein Species

Human

Activ Motif logo

Cat No : 81119

Validation Data Gallery

  • Recombinant YY1 protein gel 7.5% SDS-PAGE Coomassie staining MW: 46 kDa Purity: >85%

    Recombinant YY1 protein gel 7.5% SDS-PAGE Coomassie staining MW: 46 kDa Purity: >85%

  • HTRF assay for YY1 activity 1 uM oligo dsDNA was incubated with different concentrations of YY1 protein in for 1 hr, then FLAG antibody and SA-XL665 mixture was added to each reaction system and incubated for 30 min. HTRF assay was used for detection.

    HTRF assay for YY1 activity 1 uM oligo dsDNA was incubated with different concentrations of YY1 protein in for 1 hr, then FLAG antibody and SA-XL665 mixture was added to each reaction system and incubated for 30 min. HTRF assay was used for detection.

Product Information

Expressed In Baculovirus
Protein Species Human

Contents

Recombinant YY1 protein is supplied at a concentration of 0.3 ug/ul in 25 mM HEPES pH 7.5, 300 mM NaCl, 10% glycerol, 0.04% Triton X-100 and 0.5 mM TCEP.

Background

YY1 (YY1 Transcription Factor, also known as INO80S) is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. YY1 transcription factor,repressor/activator (Yin Yang) protein, is a multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. It binds to the consensus sequence 5’-CCGCCATNTT-3’; some genes have been shown to contain a longer binding motif allowing enhanced binding; the initial CG dinucleotide can be methylated greatly reducing the binding affinity. The effect on transcription regulation is depending upon the context in which it binds and diverse mechanisms of action include direct activation or repression, indirect activation or repression via cofactor recruitment, or activation or repression by disruption of binding sites or conformational DNA changes. Its activity is regulated by transcription factors and cytoplasmic proteins that have been shown to abrogate or completely inhibit YY1-mediated activation or repression. For example, it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence. YY1 is also involved in DNA repair. In vitro, it binds to DNA recombination intermediate structures (Holliday junctions).

Application Notes

This protein is suitable for use in protein-protein interaction, in vitro transcription assay, binding assay. Binding Assay Condition: 1 uM oligo ds DNA (DNA sequence 5’-GCGCCGCCATCTTCAT-3’) was incubated with different concentrations of YY1 protein in binding system for 1 hour, then FLAG antibody and SA-XL665 mixture was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.

Protein Details

Recombinant human YY1 protein was expressed in a baculovirus expression system as the full length protein (accession number NP_003394.1) with an N-terminal FLAG tag. The molecular weight of the protein is 46 kDa.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.