Recombinant YEATS2 (200-340) protein
Expressed In
E. coli
Protein Species
Human
Cat No : 81099
Validation Data Gallery
Product Information
| Expressed In | E. coli |
| Protein Species | Human |
Contents
Recombinant YEATS2 (200-340) protein is supplied in 25 mM Tris-HCl pH 8.0, 300 mM NaCl, 10% glycerol and 0.5 mM TCEP.
Background
YEATS2 (YEATS Domain Containing 2), is a component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4. YEATS2 specifically recognizes and binds histone H3 crotonylated at ‘Lys-27’ (H3K27cr). Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors.
Application Notes
Recombinant YEATS2 (200-340) protein is suitable for use in enzyme kinetics, inhibitor screening, and selectivity profiling. Binding Assay Conditions: 3 uM H3K27cr (crotonylated Lysine 27) peptide was incubated with different concentrations of YEATS2 (200-340) protein in a 10 ul reaction system containing 50mM HEPES-NaOH pH7.5, 0.1% BSA for 1 hour, then 10 ul FLAG antibody and SA-XL665 mixture (each 1:100 dilution in Binding Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.
Protein Details
Recombinant human YEATS2 (200-340) that includes amino acids 200-340 (accession number NP_001338299.1) was expressed in E. coli cells with an N-terminal 6xHis Tag and a C-terminal Flag tag. The molecular weight of the protein is 21.5 kDa.
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.