• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Recombinant USP7 protein

Expressed In

Baculovirus

Protein Species

Human

Activ Motif logo

Cat No : 31525

Validation Data Gallery

  • Recombinant USP7 protein gel 8% SDS-PAGE gel stained with Coomassie Blue MW: 131.3 kDa
Purity: >80%

    Recombinant USP7 protein gel 8% SDS-PAGE gel stained with Coomassie Blue MW: 131.3 kDa
Purity: >80%

  • DUB-Detector assay (Cat. No. 40110) for USP7 protein activity 100 nM Fluorescent Substrate was incubated with different concentration of USP7 protein for 1 hour at room temperature. The fluorescent intensity of the reaction was measured with an excitation wavelength of 485 nm and an emission wavelength of 535nm.

    DUB-Detector assay (Cat. No. 40110) for USP7 protein activity 100 nM Fluorescent Substrate was incubated with different concentration of USP7 protein for 1 hour at room temperature. The fluorescent intensity of the reaction was measured with an excitation wavelength of 485 nm and an emission wavelength of 535nm.

  • DUB-Detector assay (Cat. No. 40110) for USP7 protein activity 100 nM Fluorescent Substrate was incubated with different concentration of USP7 protein for 1 hour at room temperature. The fluorescent intensity of the reaction was measured with an excitation wavelength of 485 nm and an emission wavelength of 535nm.

    DUB-Detector assay (Cat. No. 40110) for USP7 protein activity 100 nM Fluorescent Substrate was incubated with different concentration of USP7 protein for 1 hour at room temperature. The fluorescent intensity of the reaction was measured with an excitation wavelength of 485 nm and an emission wavelength of 535nm.

Product Information

Expressed In Baculovirus
Protein Species Human

Contents

Recombinant full length USP7 protein is supplied in 25 mM Tris-HCl pH 8.0, 300 mM NaCl, 5% glycerol, 0.04% Triton X-100.

Background

USP7 (Ubiquitin Specific Peptidase 7 (Herpes Virus-Associated)) belongs to the peptidase C19 family, which includes ubiquitinyl hydrolases. USP7 de-ubiquitinates target proteins such as FOXO4, p53/TP53, MDM2, UHRF1, PTEN and DAXX. Together with DAXX, USP7 prevents MDM2 self-ubiquitination and enhances the E3 ligase activity of MDM2 towards p53/TP53, thereby promoting p53/TP53 ubiquitination and proteasomal degradation. USP7 de-ubiquitinates p53/TP53, preventing degradation of p53/TP53, and enhances p53/TP53-dependent transcription regulation, cell growth repression and apoptosis. USP7 also de-ubiquitinates FOXO4 and inhibits FOXO4-induced transcriptional activity. Together with DAXX, USP7 is involved in the de-ubiquitination and translocation of PTEN from the nucleus to the cytoplasm, both processes that are counteracted by PML. USP7 de-ubiquitinates UHRF1, which prevents its degradation and promoting DNA methylation by DNMT1.

Application Notes

This protein is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Protein Details

Recombinant human USP7 was expressed in a baculovirus expression system as the full length protein (accession number NP_003461.2) with an N-terminal 6xHis Tag. The molecular weight of the protein is 131.3 kDa. The purity of USP7 protein is >80% by SDS-PAGE.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.