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Recombinant PRMT6 protein

Expressed In

Baculovirus

Protein Species

Human

Activ Motif logo

Cat No : 31394

Validation Data Gallery

  • Recombinant PRMT6 protein gel. PRMT6 protein was run on a 10% SDS-PAGE gel and stained with Coomassie blue.

    Recombinant PRMT6 protein gel. PRMT6 protein was run on a 10% SDS-PAGE gel and stained with Coomassie blue.

  • PRMT6 activity assay. 30 ng/ul Recombinant Histone H3.1 (Cat# 31294) or 60 ng/ul Calf Thymus Octamer were incubated with 15 ng/ul PRMT6 (+) and 30 ng/ul PRMT6(++), respectively, in reaction buffer for 3 hours at room temperature. Activity was detected by fluorography.

    PRMT6 activity assay. 30 ng/ul Recombinant Histone H3.1 (Cat# 31294) or 60 ng/ul Calf Thymus Octamer were incubated with 15 ng/ul PRMT6 (+) and 30 ng/ul PRMT6(++), respectively, in reaction buffer for 3 hours at room temperature. Activity was detected by fluorography.

Product Information

Expressed In Baculovirus
Protein Species Human

Contents

Full length recombinant PRMT6 protein was expressed in Sf9 cells and is supplied in 25 mM Hepes pH 7.5, 300 mM NaCl, 5% glycerol, 0.04% Triton X-100, 0.2 mM TCEP.

Background

PRMT6 (Protein Arginine Methyltransferase 6, HRMT1L6, ANM6) is a type I arginine methyltransferase. Arginine methylation is a common post-translational modification of histones and other cellular proteins. PRMT6 preferentially methylates arginine residues present in GAR (glycine and arginine-rich) domains. PRMT6 asymmetrically dimethylates histone H3 at Arg2, which is mutually exclusive to methylation at lysine 4 (associated with transcriptional activation), and thus PRMT6 serves as a transcriptional repressor in this context. It can also symmetrically dimethylate H2A and H4 at Arg3 and serves as a coactivator of transcription in concert with nuclear receptors.

Application Notes

Recombinant PRMT6 is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. HMT Assay Conditions:60 ng/ul Calf Thymus Octamers were incubated with 30 ng/ul recombinant PRMT6 protein in reaction buffer including 50 mM Tris-HCl, pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 9 nCi/μl 3H-SAM for 3 hours at room temperature. Activity was detected by fluorography.

Protein Details

Recombinant PRMT6 (accession number NP_060607.2) was expressed in Sf9 cells and contains an N-terminal FLAG-Tag with an observed molecular weight of 43.7 kDa. This recombinant protein is ≥ 85% pure by SDS-PAGE.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.