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Recombinant PLK3 (57-340) protein

Expressed In

Baculovirus

Protein Species

Human

Activ Motif logo

Cat No : 81372

Validation Data Gallery

  • Recombinant PLK3 (57-340) protein 10% SDS-PAGE gel with Coomassie blue staining MW: 33.7 kDa Purity: >85%

    Recombinant PLK3 (57-340) protein 10% SDS-PAGE gel with Coomassie blue staining MW: 33.7 kDa Purity: >85%

  • HTRF assay for PLK3 (57-340) activity 1 uM STK S3 substrate was incubated with different concentrations of PLK3 (57-340) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 2 mg/ml CaSein and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.

    HTRF assay for PLK3 (57-340) activity 1 uM STK S3 substrate was incubated with different concentrations of PLK3 (57-340) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 2 mg/ml CaSein and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.

  • HTRF assay for PLK3 (57-340) activity 1 uM STK S3 substrate was incubated with different concentrations of PLK3 (57-340) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 2 mg/ml CaSein and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.

    HTRF assay for PLK3 (57-340) activity 1 uM STK S3 substrate was incubated with different concentrations of PLK3 (57-340) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 2 mg/ml CaSein and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.

Product Information

Expressed In Baculovirus
Protein Species Human

Contents

Recombinant PLK3 (57-340) protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10% glycerol, 0.04% Triton X-100, and 0.5 mM TCEP.

Background

PLK3 (Polo Like Kinase 3) is a serine/threonine-protein kinase involved in cell cycle regulation, response to stress and Golgi disassembly and phosphorylates ATF2, BCL2L1, CDC25A, CDC25C, CHEK2, HIF1A, JUN, p53/ TP53, p73/TP73, PTEN, TOP2A and VRK1. PLK3 phosphorylates BCL2L1, leading to regulate the G2 checkpoint and progression to cytokinesis during mitosis and plays a key role in response to stress: rapidly activated upon stress stimulation, such as ionizing radiation, reactive oxygen species (ROS), hyperosmotic stress, UV irradiation and hypoxia. PLK3 involved in DNA damage response and G1/S transition checkpoint by phosphorylating CDC25A, p53/TP53 and p73/TP73 and phosphorylates p53/TP53 in response to reactive oxygen species (ROS), thereby promoting p53/TP53- mediated apoptosis and phosphorylates CHEK2 in response to DNA damage, promoting the G2/M transition checkpoint. It phosphorylates the transcription factor p73/TP73 in response to DNA damage, leading to inhibit p73/TP73-mediated transcriptional activation and pro-apoptotic functions and phosphorylates HIF1A and JUN is response to hypoxia and phosphorylates ATF2 following hyperosmotic stress in corneal epithelium. Diseases associated with PLK3 include Breast Cancer.

Application Notes

This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.

Protein Details

Recombinant PLK3 (57-340) protein that includes amino acids 57-340 of human PLK3 protein (accession number NP_004064.2) was expressed in a baculovirus expression system, and contains an N-terminal FLAG tag. The molecular weight of the protein is 33.7 kDa.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.