Recombinant PDK1 protein, GST-Tag

Expressed In

Baculovirus

Protein Species

Human

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Cat No : 81213



Product Information

Expressed In Baculovirus
Protein Species Human

Contents

Recombinant PDK1 protein, GST-Tag is supplied in 25 mM Tris-HCl pH 8.0, 300 mM NaCl, 10% glycerol, 0.04% Triton X-100 and 0.5 mM TCEP.

Background

PDK1 (Pyruvate Dehydrogenase Kinase 1), also known as PDHK1, is a serine/threonine kinase. It plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. PDK1 plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress. Its activity can be enhanced by binding to the pyruvate dehydrogenase subunit DLAT, inhibited by AZD7545 (this compound interferes with DLAT binding and thereby inhibits kinase activity). Its activity can also be inhibited by dichloroacetate and radicicol.

Application Notes

Recombinant PDK1 protein, GST-Tag is suitable for use in binding assays, inhibitor screening, and selectivity profiling.

Protein Details

Recombinant PDK1 protein, GST-Tag was expressed in baculovirus expression system as the full length protein (accession number NP_002604.1) with an N-terminal GST tag. The molecular weight of the protein is 89.8 kDa.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.