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Recombinant OGT protein

Expressed In

Baculovirus

Protein Species

Mouse

Activ Motif logo

Cat No : 31524

Validation Data Gallery

  • Recombinant OGT protein gel. OGT protein was run on an 8% SDS-PAGE gel and stained with Coomassie Blue.

    Recombinant OGT protein gel. OGT protein was run on an 8% SDS-PAGE gel and stained with Coomassie Blue.

Product Information

Expressed In Baculovirus
Protein Species Mouse

Contents

Recombinant full length Mouse OGT protein is supplied in 25 mM HEPES pH 7.5, 300 mM NaCl, 5% glycerol, 0.04% Triton X-100, 0.2 mM TCEP.

Background

OGT (O-linked N-acetylglucosamine (GlcNAc) transferase) catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to serine or threonine residues in cytoplasmic and nuclear proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc). OGT can glycosylate a large and diverse number of proteins including HCF1, AKT1, MLL5 and histone H2B. It can regulate their cellular processes via cross-talk between glycosylation and phosphorylation or by affecting proteolytic processing. Specifically OGT has been shown to interact directly with TET2 and TET3, enzymes that catalyze the oxidation of 5-methylcytosine on DNA. The TET/OGT interaction leads to GlcNAcetylation of HCF1, a protein component of the COMPASS complex, which regulates H3K4 methylation and gene expression.

Application Notes

This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.

Protein Details

Recombinant mouse OGT was expressed in a baculovirus expression system as the full length protein (accession number NP_001277464.1) with an N-terminal FLAG-Tag. The molecular weight of the protein is 118.7 kDa. The purity of OGT protein is >90 by SDS-PAGE.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.