Recombinant Mononucleosomes H3K27ac

Expressed In

E. coli / Synthetic

Protein Species

Human

Cat No : 81077

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Validation Data Gallery

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  Recombinant Mononucleosomes (H3K27ac) DNA gel. Recombinant Mononucleosomes (H3K27ac) were run on a 2% agarose gel and stained with ethidium bromide. Lane 1: DNA marker. Lane 2: 601 DNA. Lane 3: Intact mononucleosomes H3K27ac. Intact mononucleosomes H3K27ac migrated much higher than free 601 DNA. The agarose gel result shows almost all of 601 DNA wrap histone octamers to form nucleosomes.

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  Recombinant Mononucleosomes (H3K27ac) gel. 13% SDS-PAGE Coomassie staining Purity: ≥95%

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  Western Blot analysis for Recombinant Mono-nucleosomes (H3K27ac) Recombinant Mononucleosomes (H3K27ac) (Lane 1) and unmodified nucleosomes (Lane 2) were detected with anti- H3K27ac antibody and anti-H4 antibody, respectively. H4 was detected as loading control. Only Recombinant Mononucleosomes (H3K27ac) can be detected by anti-H3K27ac antibody.

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Product Information

• Expressed In: E. coli / Synthetic
• Protein Species: Human

Contents

Recombinant Mononucleosomes (H3K27ac) (10 ug protein + 10 ug DNA) is supplied in 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 2 mM DTT and 20% glycerol.

Background

In vivo, histones are wrapped around by DNA in chromatin. Therefore, nucleosomes are more physiologically relevant substrates than histones and histone-derived peptides for in vitro studies. More importantly, some histone methyltransferases are significantly more active, as well as specific, when using nucleosomal substrates in HMT assays, such as DOT1L and NSD family enzymes. Nucleosomes are also widely used in histone methyltransferase screening assays to identify small molecular inhibitors for drug discovery.

Application Notes

Recombinant Mononucleosomes (H3K27ac) are suitable for use as substrate of enzymatic assay or other biochemical assay.

Protein Details

Recombinant Mononucleosomes (H3K27ac) consist of a 167 bp of 601 DNA and two molecules each of histones H2A that includes amino acids 1-130 (end) (accession number NP_003503.1), H2B that includes amino acids 1-126 (end) (accession number NP_003509.1), H3.1 that includes amino acids 1-136 (end) (accession number NP_003520.1) with acetylation at lysine 27, and H4 that includes amino acids 1-103 (end) (accession number NP_003539.1). Histone H2A, H2B and H4 were expressed in E. coli cells. H3K27ac was synthesized completely. Histone H3K27ac corresponds to the native histone sequence and does not contain any amino acid substitutions or residue analogs. The molecular weight of histone octamer is ~108 kDa.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.