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Recombinant KAT6B / MORF (718-1008) protein

Expressed In

Baculovirus

Protein Species

Human

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Cat No : 81224

Validation Data Gallery

  • Recombinant KAT6B / MORF (718-1008) protein gel 10% SDS-PAGE gel with Coomassie blue staining MW: 35.7 kDa Purity: > 93%

    Recombinant KAT6B / MORF (718-1008) protein gel 10% SDS-PAGE gel with Coomassie blue staining MW: 35.7 kDa Purity: > 93%

  • Recombinant KAT6B / MORF (718-1008) protein activity assay 0.5 ug Histone H3.1 (Cat. No. 31296) was incubated with 0 ug (-), 0.1 ug (+), 0.2 ug (++), 0.4 ug (+++) KAT6B / MORF (718-1008) protein in a 20 ul reaction system containing 50 mM Tris-HCl pH 8.6, 2 mM MgCl2, 1 mM TCEP, 0.02% Triton X-100 and 20 uM Acetyl-CoA for 2 hr at RT. Reaction products were detected by Western blot using H3ac (pan-acetyl) antibody (Cat. No. 61637).

    Recombinant KAT6B / MORF (718-1008) protein activity assay 0.5 ug Histone H3.1 (Cat. No. 31296) was incubated with 0 ug (-), 0.1 ug (+), 0.2 ug (++), 0.4 ug (+++) KAT6B / MORF (718-1008) protein in a 20 ul reaction system containing 50 mM Tris-HCl pH 8.6, 2 mM MgCl2, 1 mM TCEP, 0.02% Triton X-100 and 20 uM Acetyl-CoA for 2 hr at RT. Reaction products were detected by Western blot using H3ac (pan-acetyl) antibody (Cat. No. 61637).

Product Information

Expressed In Baculovirus
Protein Species Human

Contents

Recombinant KAT6B / MORF (718-1008) protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10% glycerol, 0.04% Triton X-100, 0.5 mM TCEP.

Background

KAT6B (Lysine Acetyltransferase 6B), also called as MOZ2, MORF or MYST4, is a histone acetyltransferase and component of the MOZ/MORF protein complex. It has a histone H3 acetyltransferase activity. In addition to its acetyltransferase activity, the encoded full length protein has transcriptional activation activity in its N-terminal end and transcriptional repression activity in its C-terminal end. So it may be involved in both positive and negative regulation of transcription. KAT6B is necessary for RUNX2-dependent transcriptional activation and could be involved in brain development. Mutations have been found in patients with genitopatellar syndrome. A translocation of KAT6B gene and the CREBBP gene results in acute myeloid leukemias. It may also be involved in cerebral cortex development.

Application Notes

Recombinant KAT6B / MORF (718-1008) protein is suitable for use in enzyme kinetics, inhibitor screening, and selectivity profiling.

Protein Details

Recombinant KAT6B / MORF (718-1008) protein that includes amino acids 718-1008 of human KAT6B protein (accession number NP_036462.2) was expressed in a baculovirus expression system and contains an N-terminal FLAG tag. The molecular weight of the protein is 35.7 kDa. This product is the catalytic domain of KAT6B protein.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.