Recombinant JMJD3 / KDM6B (1043-1682) protein

Expressed In

Baculovirus

Protein Species

Human

Cat No : 31519

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Validation Data Gallery

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  Recombinant JMJD3 / KDM6B (1043-1682) SDS-PAGE gel. Recombinant JMJD3 was run on an 8% SDS-PAGE gel and stained with Coomassie Blue. MW: 73.4 kDa Purity: > 90%

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  HTRF assay for JMJD3 / KDM6B (1043-1682) activity.1 uM histone H3K27me3 peptide was incubated with JMJD3 (1043-1682) protein in reaction buffer for 2 hours at room temperature. Reaction products were detected with H3K27me2 antibody.

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  HTRF assay for JMJD3 / KDM6B (1043-1682) activity.1 uM histone H3K27me3 peptide was incubated with JMJD3 (1043-1682) protein in reaction buffer for 2 hours at room temperature. Reaction products were detected with H3K27me2 antibody.

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Product Information

• Expressed In: Baculovirus
• Protein Species: Human

Contents

Recombinant JMJD3 / KDM6B (1043-1682) protein is supplied at a concentration of 0.4 ug/ul in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 5% glycerol, 0.04% Triton X-100, 0.2 mM TCEP.

Background

JMJD3 / KDM6B (Jumonji domain-containing protein 3 lysine (K)-specific demethylase 6B) is a histone demethylase that specifically demethylates ‘Lys-27’ of histone H3, thereby, playing a central role in histone code. JMJD3 demethylates trimethylated and dimethylated H3 ‘Lys-27’. It plays a central role in regulation of posterior development by regulating HOX gene expression. JMJD3 / KDM6B is involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation.

Application Notes

Recombinant JMJD3 / KDM6B (1043-1682) is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. HDM Activity Assay Conditions: 1 uM H3K27me3 peptide was incubated with different concentrations of JMJD3 (1043- 1682) protein in 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.4, 0.02% Triton X-100, 1 mM TCEP, 50 uM 2-OG, 50 uM Ascorbate and 25 uM (NH4)2Fe(SO4)2·6H2O for 2 hours, then 10 micro;l H3K27me2 antibody and SA-XL665 mixture (each 1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.

Protein Details

Recombinant JMJD3 / KDM6B (1043-1682) that includes amino acids 1043 - 1682 of human JMJD3 / KDM6B protein (accession number NP_001073893.1) was expressed in Baculovirus expression system and contains a C-terminal Flag tag with a molecular weight of 73.4 kDa. The recombinant protein is ≥ 90% pure by SDS-PAGE.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.