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Recombinant JMJD2D / KDM4D protein

Expressed In

Baculovirus

Protein Species

Human

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Cat No : 31459

Validation Data Gallery

  • Recombinant JMJD2D / KDM4D protein gel. JMJD2D / KDM4D protein was run on an SDS-PAGE gel and stained with Coomassie Blue.

    Recombinant JMJD2D / KDM4D protein gel. JMJD2D / KDM4D protein was run on an SDS-PAGE gel and stained with Coomassie Blue.

  • MALDI-TOF Assay for JMJD2D / KDM4D activity . 3.3 uM H3K9me3 peptide was incubated with 100 nM JMJD2D / KDM4D in reaction buffer for 2 hours at room temperature. The reaction product was detected by MALDI-TOF. Single 3.3 uM H3K9me3 peptide was used as a negative control.Catalytic Ability: >66 turnovers / enzyme molecule.

    MALDI-TOF Assay for JMJD2D / KDM4D activity . 3.3 uM H3K9me3 peptide was incubated with 100 nM JMJD2D / KDM4D in reaction buffer for 2 hours at room temperature. The reaction product was detected by MALDI-TOF. Single 3.3 uM H3K9me3 peptide was used as a negative control.Catalytic Ability: >66 turnovers / enzyme molecule.

  • Recombinant JMJD2D / KDM4D activity using AlphaLISA. JMJD2D / KDM4D was used in an AlphaLISA assay to determine enzyme titration. An IC50 dose response assessment of reference compounds 2,4-PDCA and N-OG is shown. This data was generated and kindly provided courtesy of ChemPartner.

    Recombinant JMJD2D / KDM4D activity using AlphaLISA. JMJD2D / KDM4D was used in an AlphaLISA assay to determine enzyme titration. An IC50 dose response assessment of reference compounds 2,4-PDCA and N-OG is shown. This data was generated and kindly provided courtesy of ChemPartner.

Product Information

Expressed In Baculovirus
Protein Species Human

Contents

Full length recombinant JMJD2D / KDM4D protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 5% glycerol, 0.04% Triton X-100, 0.2 mM TCEP.

Background

KDM4D (lysine (K)-specific demethylase 4D), also known as JMJD2D (Jumonji Domain Containing 2D) is a protein that functions as a histone demethylase that preferentially demethylates di- and trimethylated lysine 9 residues of histone H3, while it has no activity on monomethylated H3K9 residues.

Application Notes

Recombinant JMJD2D / KDM4D is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Histone Demethylase Assay Conditions: 50 mM HEPES pH 7.5, 0.02% Triton X100, 100 μM 2OG, 100 μM Ascorbate, 50 μM (NH4)2Fe(SO4)2·6H2O, 1 mM TCEP, 100 nM Recombinant JMJD2D / KDM4D protein, and 3.3 μM H3K9me3 (aa 1-21) peptide at 2 hours at room temperature. MALDI-TOF was used for detection.

Protein Details

Recombinant JMJD2D / KDM4D (accession number NP_060509.2) was expressed in Sf9 cells and contains an N-terminal FLAG-Tag with an observed molecular weight of 63.2 kDa. The recombinant protein is >60% pure by SDS-PAGE.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.