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Recombinant HDAC1 protein

Expressed In

Baculovirus

Protein Species

Human

Activ Motif logo

Cat No : 31504

Validation Data Gallery

  • Recombinant HDAC1 protein gel. HDAC1 protein was run on a 10% SDS-PAGE gel and stained with Coomassie blue. MW: 56 kDa Purity> >65%

    Recombinant HDAC1 protein gel. HDAC1 protein was run on a 10% SDS-PAGE gel and stained with Coomassie blue. MW: 56 kDa Purity> >65%

  • Recombinant HDAC1 activity by HTRF Assay. 1 μM H3K9ac (1-21aa.) peptide was incubated with different concentrations of HDAC1 protein in 10 μl reaction system containing 25 mM Tris-HCl pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 0.1 mg/ml BSA for 30 min at room temperature, then 10 μl anti-H3K9me0 antibody and SA-XL665 mixture (1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min at room temperature. All operations and reactions were performed at room temperature. HTRF assay was used for detection.

    Recombinant HDAC1 activity by HTRF Assay. 1 μM H3K9ac (1-21aa.) peptide was incubated with different concentrations of HDAC1 protein in 10 μl reaction system containing 25 mM Tris-HCl pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 0.1 mg/ml BSA for 30 min at room temperature, then 10 μl anti-H3K9me0 antibody and SA-XL665 mixture (1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min at room temperature. All operations and reactions were performed at room temperature. HTRF assay was used for detection.

  • Recombinant HDAC1 activity by HTRF Assay. 1 μM H3K9ac (1-21aa.) peptide was incubated with different concentrations of HDAC1 protein in 10 μl reaction system containing 25 mM Tris-HCl pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 0.1 mg/ml BSA for 30 min at room temperature, then 10 μl anti-H3K9me0 antibody and SA-XL665 mixture (1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min at room temperature. All operations and reactions were performed at room temperature. HTRF assay was used for detection.

    Recombinant HDAC1 activity by HTRF Assay. 1 μM H3K9ac (1-21aa.) peptide was incubated with different concentrations of HDAC1 protein in 10 μl reaction system containing 25 mM Tris-HCl pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 0.1 mg/ml BSA for 30 min at room temperature, then 10 μl anti-H3K9me0 antibody and SA-XL665 mixture (1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min at room temperature. All operations and reactions were performed at room temperature. HTRF assay was used for detection.

Product Information

Expressed In Baculovirus
Protein Species Human

Contents

Full length recombinant HDAC1 protein was expressed in Sf9 cells and is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10% glycerol, 0.04% Triton X-100, and 0.5 mM TCEP.

Background

HDAC1 (Histone Deacetylase 1, also designated HD1) is a member of the class I mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-e-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the e-amino group of a lysine residue. In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: HDAC class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, HDAC9 and HDAC10), class III (SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6 and SIRT-7) and class IV (HDAC11). HDAC1 and HDAC2 are recruited to Mad-Max complexes, which associate with the mSin3 scaffold protein, and are required for the transcriptional repression of Mad-Max target genes. HDAC1 is also involved in the regulation of p53. HDAC1 is expressed in various tissues. HDAC1, HDAC2 and HDAC3 are also ubiquitously expressed and can deacetylate both Histone H3 and Histone H4 in free histones or nucleosome substrate.

Application Notes

This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.

Protein Details

Recombinant human HDAC1 was expressed in Sf9 cells as the full length protein (accession number NP_004955.2) with a C-terminal FLAG-Tag. The molecular weight of the protein is 56 kDa. The recombinant protein is > 65% pure by SDS-PAGE.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.