Recombinant DNMT3A / DNMT3L complex
Expressed In
Baculovirus
Protein Species
Human
Cat No : 31415
Validation Data Gallery
Product Information
| Expressed In | Baculovirus |
| Protein Species | Human |
Contents
Recombinant DNMT3A / DNMT3L complex is supplied in 25 mM Hepes pH 7.5, 300 mM NaCl, 5% Glycerol, 0.04% Triton X-100, 0.2 mM TCEP and 0.2 mg/ml 3× Flag peptide. Please refer to product insert upon arrival for lot-specific concentration.
Background
DNA methyltransferase proteins such as DNMT3A are involved in DNA methylation, in which a methyl group is added to a cytosine residue on DNA, commonly at the C5 position of a CpG dinucleotide. DNMT3A has been shown to be important in the regulation of specific patterns of DNA methylation. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as embryonic development, genetic imprinting, X chromosome inactivation and control of gene expression. DNA methylation is generally associated with transcriptional repression. Three families of DNMTs have been identified: DNMT1, DNMT2 and DNMT3. The DNMT3 family, containing two active methyltransferases, DNMT3A & DNMT3B, and one DNMT3-Like protein (DNMT3L), establishes the initial CpG methylation pattern de novo and shows the same propensity for methylating unmethylated duplex DNA as for hemi-methylated DNA. DNMT3A and DNMT3B are encoded by two distinct genes and are strongly expressed in embryonic stem cells. DNMT3A is ubiquitous in mammalian cells. DNMT3L is a nuclear protein with similarity to the DNMT family of proteins, but does not function as a DNA methyltransferase. It serves as an essential regulator of DNMT3A and DNMT3B, recruiting them to sites for deposition of de novo DNA methylation.
Application Notes
Recombinant DNMT3A / DNMT3L complex is suitable for use in enzyme kinetics, inhibitor screening, and selectivity profiling. DNMT Activity Assay Conditions: Assay was performed using DNMT Activity / Inhibition Assay, Active Motif Catalog No. 55006, for 1 hour at 37°C.
Protein Details
Recombinant DNMT3A / DNMT3L complex that includes human DNMT3A amino acids 214-912 (accession number NP_783328.1) with an N-terminal 6xHis Tag and FLAG-Tag and full length DNMT3L (accession number NP_787063.1) with an N-terminal 6xHis Tag was expressed in Sf9 cells. The molecular weights of DNMT3A and DNMT3L are 83.8 kDa and 46.6 kDa, respectively. The recombinant protein is >95% pure by SDS-PAGE.
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.