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Recombinant AURKB protein

Expressed In

Baculovirus

Protein Species

Human

Activ Motif logo

Cat No : 81352

Validation Data Gallery

  • Recombinant AURKB protein gel 10% SDS-PAGE with Coomassie staining MW: 40.5 kDa Purity: >80%

    Recombinant AURKB protein gel 10% SDS-PAGE with Coomassie staining MW: 40.5 kDa Purity: >80%

  • HTRF assay for AURKB activity 1 uM STK S2 substrate was incubated with different concentrations of AURKB protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100 μM ATP for 1 hour. The 10 ul detection reagents containing STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.

    HTRF assay for AURKB activity 1 uM STK S2 substrate was incubated with different concentrations of AURKB protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100 μM ATP for 1 hour. The 10 ul detection reagents containing STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.

  • HTRF assay for AURKB activity 1 uM STK S2 substrate was incubated with different concentrations of AURKB protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100 μM ATP for 1 hour. The 10 ul detection reagents containing STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.

    HTRF assay for AURKB activity 1 uM STK S2 substrate was incubated with different concentrations of AURKB protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100 μM ATP for 1 hour. The 10 ul detection reagents containing STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.

Product Information

Expressed In Baculovirus
Protein Species Human

Contents

Recombinant AURKB protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10% glycerol, 0.04% Triton X-100, and 0.5 mM TCEP.

Background

AURKB (Aurora Kinase B) is a member of the aurora kinase subfamily of serine/threonine kinases and phosphorylates the CPC complex subunits BIRC5/survivin, CDCA8/borealin and INCENP. Phosphorylation of INCENP leads to increased AURKB activity. The CPC complex acts as a key regulator of mitosis. which has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatininduced microtubule stabilization and spindle assembly. Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis. Other known AURKB substrates involved in centromeric functions and mitosis are CENPA, DES/desmin, GPAF, KIF2C, NSUN2, RACGAP1, SEPTIN1, VIM/ vimentin, HASPIN, and histone H3. Phosphorylation of VIM controls vimentin filament segregation in cytokinetic process, whereas histone H3 is phosphorylated at ‘Ser-10’ and ‘Ser-28’ during mitosis (H3S10ph and H3S28ph, respectively). A positive feedback between HASPIN and AURKB contributes to CPC localization. AURKB is also required for kinetochore localization of BUB1 and SGO1.

Application Notes

This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data this product is shown.

Protein Details

Recombinant AURKB protein was expressed in baculovirus expression system as the full length protein (accession number AAC12709.1) with a N-terminal FLAG tag. The molecular weight of the protein is 40.5 kDa.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.