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RNA pol II CTD phospho Ser7 antibody (mAb) (Clone 4E12)

Host / Isotype

Rat / IgG1

Reactivity

Human

Applications

ChIP, ChIP-Seq, ICC, IF, IP, WB

CloneNo.

4E12

Activ Motif logo

Cat No : 61087,61088,61987 61087

Synonyms

RNA Pol II CTD, RNA Pol II, RNA polII, CTD, pAb, polyclonal, RNA polymerase II, antibody, antibodies, sample, monoclonal, clone 4E12, C terminal domain, heptad repeat, phospho ser7, serine 7, serine7, sample

Validation Data Gallery

  • RNA pol II CTD phospho Ser7 antibody (mAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with chromatin from 2.3 million HL-60 cells and 4 ul of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 20 million sequence tags were mapped to identify Pol II phospho Ser7 binding. The image shows a 350,000 base pair region on chromosome 1 with the expected enrichment at gene promoters.

    RNA pol II CTD phospho Ser7 antibody (mAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with chromatin from 2.3 million HL-60 cells and 4 ul of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 20 million sequence tags were mapped to identify Pol II phospho Ser7 binding. The image shows a 350,000 base pair region on chromosome 1 with the expected enrichment at gene promoters.

Product Information

Tested Applications ChIP, ChIP-Seq, ICC, IF, IP, WB

Applications Validated by Active Motif: ChIP: 5-10 ug per ChIP ChIP-Seq: 5-10 ug each
Tested Reactivity Human
Host / Isotype Rat / IgG1
Class Monoclonal
Type Antibody
ModificationPhosphorylated
Immunogen This RNA pol II CTD phospho Ser7 antibody was raised against a peptide containing the RNA pol II CTD sequence phosphorylated at serine 7.
Full Name RNA pol II CTD phospho Ser7 antibody (mAb) (Clone 4E12)
Synonyms RNA Pol II CTD, RNA Pol II, RNA polII, CTD, pAb, polyclonal, RNA polymerase II, antibody, antibodies, sample, monoclonal, clone 4E12, C terminal domain, heptad repeat, phospho ser7, serine 7, serine7, sample
Molecular weight 240 kDa
GenBank accession numberNP_000928
RRIDAB_2687452
Purification Method Protein G Chromatography
Buffer Purified IgG in PBS, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. During the transcription cycle, the CTD of the large subunit of RNA pol II is reversibly phosphorylated. RNA pol II containing unphosphorylated CTD is recruited to the promoter, whereas the hyperphosphorylated CTD form is involved in active transcription. Phosphorylation occurs at two sites within the heptapeptide repeat, at serine 2, serine 5 and serine 7. RNA pol II Serine 7 phosphorylation is confined to promoter regions and is necessary for the initiation of transcription.