PRMT5 antibody (pAb)
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
ChIP-Seq, WB
Cat No : 61001,61002 61001
Synonyms
Validation Data Gallery
Product Information
| Tested Applications |
ChIP-Seq, WB
Applications Validated by Active Motif: WB: 1:500 - 1:2,000 dilution The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required. |
| Tested Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | This PRMT5 antibody was raised against a peptide corresponding to amino acids within the N-terminal region of human PRMT5. |
| Full Name | PRMT5 antibody (pAb) |
| Synonyms | PRMT5, Protein Arginine Methyltransferase 1, JBP1, Janus Binding Protein 1, HRMT1L5, IBP72, JBP1, SKB1, ANM1, co-repressor, COPR5, histone H2A Arg3, H3 Arg8 and H4 Arg3, H2AR3me2s, H4R3me2s, H3R8me2s, transcription, PIWI, SNRNP, JAK2, PRDM1, RPS10, sample, prmt |
| Molecular weight | 75 kDa |
| GenBank accession number | NP_006100 |
| RRID | AB_2615010 |
| Purification Method | Affinity Purified |
| Buffer | Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| Storage | Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage. |
Background Information
PRMT5 (Protein Arginine Methyltransferase 5, also known as JBP1, Janus Binding Protein 1) is a type II arginine methyltransferase, a protein that transfers methyl groups to the arginine residues of substrate proteins. Arginine methylation is a common post-translational modification of histones and other cellular proteins. PRMT5 symmetrically dimethylates histone H2A Arg3, H3 Arg8 and H4 Arg3. It also methylates PIWI proteins which regulate small non-coding RNAs and snRNPs, regulating snRNP core particle assembly. PRMT5 functions as a co-repressor of transcription and is recruited by a number of DNA binding proteins and transcriptional repressor complexes. It is present both in the nucleus and the cytoplasm. It also interacts with COPR5, JAK2, PRDM1 and RPS10.