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PP2A antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat, Wide Range Predicted

Applications

ChIP, WB

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Cat No : 39192,39193 39192

Synonyms

PP2A, pAb, polyclonal, antibody, antibodies, chip, chromatin immunoprecipitation, sample

Validation Data Gallery

  • PP2A antibody (pAb) tested by ChIP analysis. Chromatin IP performed using Swiss 3T3 Chromatin (approximately 5 million cells treated with anisomycin and fixed at 0.5 and 3 hours) using 2.5 ul of PP2A antibody or the equivalent amount of IgG as a negative control.

    PP2A antibody (pAb) tested by ChIP analysis. Chromatin IP performed using Swiss 3T3 Chromatin (approximately 5 million cells treated with anisomycin and fixed at 0.5 and 3 hours) using 2.5 ul of PP2A antibody or the equivalent amount of IgG as a negative control.

  • PP2A pAb tested by Western blot. Nuclear extract of HeLa cells was probed with PP2A pAb (1:1,000 dilution).

    PP2A pAb tested by Western blot. Nuclear extract of HeLa cells was probed with PP2A pAb (1:1,000 dilution).

Product Information

Tested Applications ChIP, WB

Applications Validated by Active Motif: ChIP: 2 - 10 ul per ChIP WB*: 1:250 - 1:2,000 dilution> *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.
Tested Reactivity Human, Mouse, Rat, Wide Range Predicted
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen This PP2A antibody was raised against a peptide corresponding to amino acids 288-303 of human PP2A.
Full Name PP2A antibody (pAb)
Synonyms PP2A, pAb, polyclonal, antibody, antibodies, chip, chromatin immunoprecipitation, sample
Molecular weight 36 kDa
GenBank accession numberNP_821067
RRIDAB_2793178
Purification Method Affinity Purified
Buffer Peptide affinity purified IgG containing 30% glycerol, 0.035% sodium azide and 1 mg/ml BSA. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

PP2A – Protein Phosphatase 2A beta subunit is the catalytic subunit of protein phosphatase 2A. PP2A is involved in the regulation of a number of signaling pathways, and it is implicated in the negative control of cell growth and division. Mutations of PP2A occurs in many types of cancers.