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PKC-B antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB

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Cat No : 61733,61734 61733

Synonyms

PRKCB, Protein Kinase C, Beta, PRKCB1, PKCB, EC 2.7.11.13, PKC-Beta, Protein Kinase C, Beta 1 Polypeptide, Protein Kinase C Beta Type, Protein Kinase C, Beta 1, EC 2.7.11, PRKCB2, serine/threonine-protein kinase, antibody, antibodies, polyclonal, pab, western blotting, wb, sample

Validation Data Gallery

  • PKC-B antibody (pAb) tested by Western blot. PKC-B detection by Western blot. The analysis was performed using 20 ug of K562 nuclear cell extract and PKC-B antibody at a 1:500 dilution.

    PKC-B antibody (pAb) tested by Western blot. PKC-B detection by Western blot. The analysis was performed using 20 ug of K562 nuclear cell extract and PKC-B antibody at a 1:500 dilution.

Product Information

Tested Applications WB

Applications Validated by Active Motif: WB*: 1:500 - 1:2,000 dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.
Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen This antibody was raised against a peptide within the C-terminal region of human PKC-B.
Full Name PKC-B antibody (pAb)
Synonyms PRKCB, Protein Kinase C, Beta, PRKCB1, PKCB, EC 2.7.11.13, PKC-Beta, Protein Kinase C, Beta 1 Polypeptide, Protein Kinase C Beta Type, Protein Kinase C, Beta 1, EC 2.7.11, PRKCB2, serine/threonine-protein kinase, antibody, antibodies, polyclonal, pab, western blotting, wb, sample
Molecular weight 85 kDa
GenBank accession numberNP_997700
RRIDAB_2793748
Purification Method Affinity Purified
Buffer Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

PKC-B (Protein Kinase C, Beta) ia a calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase involved in various cellular processes such as regulation of the B-cell receptor (BCR) signalosome, oxidative stress-induced apoptosis, androgen receptor-dependent transcription regulation, insulin signaling and endothelial cells proliferation. Plays a key role in B-cell activation by regulating BCR-induced NF-kappa-B activation. Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11/CARMA1 at Ser-559, Ser-644 and Ser-652. Plays a direct role in the negative feedback regulation of the BCR signaling, by down-modulating BTK function via direct phosphorylation of BTK at Ser-180, which results in the alteration of BTK plasma membrane localization and in turn inhibition of BTK activity. Involved in apoptosis following oxidative damage. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of Thr-6 of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 Lys-4 (H3K4me) by LSD1/KDM1A. Under high glucose in pancreatic beta-cells, is probably involved in the inhibition of the insulin gene transcription, via regulation of MYC expression. In endothelial cells, activation of PRKCB induces increased phosphorylation of RB1, increased VEGFA-induced cell proliferation, and inhibits PI3K/AKT-dependent nitric oxide synthase (NOS3/eNOS) regulation by insulin, which causes endothelial dysfunction. Phosphorylates ATF2 which promotes cooperation between ATF2 and JUN, activating transcription.