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PBRM1 antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

ChIP-Seq, WB

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Cat No : 61381,61382 61381

Synonyms

PBRM1, polybromo 1, polybromo-1D, BAF180, protein polybromo-1, PB1, Polybromo-1D, BRG1-associated factor 180, hPB1, western blotting, wb, polyclonal, pAb, antibody, sample

Validation Data Gallery

  • PBRM1 antibody (pAb) tested by ChIP-Seq.Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 30 ug of chromatin from mouse liver and 4 ug PBRM1 antibody. ChIP DNA was sequenced on the Illumina HiSeq and 3.9 million sequence tags were mapped to identify PBRM1 binding sites.

    PBRM1 antibody (pAb) tested by ChIP-Seq.Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 30 ug of chromatin from mouse liver and 4 ug PBRM1 antibody. ChIP DNA was sequenced on the Illumina HiSeq and 3.9 million sequence tags were mapped to identify PBRM1 binding sites.

  • PBRM1 antibody (pAb) tested by Western blot. Detection of PBRM1 by Western blot. The analysis was performed using 40 ug of A-431 nuclear extract (Cat. No. 36004) and PBRM1 (pAb) at a 1:1000 dilution.

    PBRM1 antibody (pAb) tested by Western blot. Detection of PBRM1 by Western blot. The analysis was performed using 40 ug of A-431 nuclear extract (Cat. No. 36004) and PBRM1 (pAb) at a 1:1000 dilution.

Product Information

Tested Applications ChIP-Seq, WB

Applications Validated by Active Motif: ChIP-Seq: 7 ul (4 ug) per ChIP WB: 1:500 - 1:1,000 dilution Addition of 0.05% Tween 20 in blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot.
Tested Reactivity Human, Mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen This antibody was raised against a peptide within the N-terminal region of human PBRM1.
Full Name PBRM1 antibody (pAb)
Synonyms PBRM1, polybromo 1, polybromo-1D, BAF180, protein polybromo-1, PB1, Polybromo-1D, BRG1-associated factor 180, hPB1, western blotting, wb, polyclonal, pAb, antibody, sample
Molecular weight 180 kDa
GenBank accession numberNP_060783
RRIDAB_2793612
Purification Method Affinity Purified
Buffer Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

PBRM1 (polybromo 1) is a component of the SWI/SNF-B (PBAF) ATP-dependent chromatin-remodeling complex. It is involved in transcriptional activation and repression of select genes by chromatin remodeling and is an integral component of complexes necessary for ligand-dependent transcriptional activation by nuclear hormone receptors. Defects in PBRM1 have been associated with primary clear cell renal cell carcinoma.