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PARP-2 antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

ChIP, IF, IP, WB

Activ Motif logo

Cat No : 39044,39743,39744 39743

Synonyms

PARP-2, poly ADP-ribosyl transferase, PPOL, DNA damage, antibody, antibodies, polyclonal, apotosis, chip, chromatin immunoprecipitation, sample

Validation Data Gallery

  • PARP-2 antibody tested by ChIP analysis. Chromatin IP performed on stably transfected AC16 cells using the per-immune serum (Pre) or the PARP-2 antibody. ChIP DNA was subjected to qPCR using primers specific for human CD36 gene. Data are presented relative to input.

    PARP-2 antibody tested by ChIP analysis. Chromatin IP performed on stably transfected AC16 cells using the per-immune serum (Pre) or the PARP-2 antibody. ChIP DNA was subjected to qPCR using primers specific for human CD36 gene. Data are presented relative to input.

  • PARP-2 antibody used for immunoprecipitation. Western blot of immunoprecipitation using the PARP-2 antibody. Lane 1: input (AC16 whole cell lystate). Lane 2: IP using pre-immune serum. Lane 3: IP using PARP-2 antibody.

    PARP-2 antibody used for immunoprecipitation. Western blot of immunoprecipitation using the PARP-2 antibody. Lane 1: input (AC16 whole cell lystate). Lane 2: IP using pre-immune serum. Lane 3: IP using PARP-2 antibody.

  • PARP-2 antibody tested by Western blot. HeLa cell nuclear extract (20 ug) was probed with PARP-2 antibody at a dilution of 1:1000.

    PARP-2 antibody tested by Western blot. HeLa cell nuclear extract (20 ug) was probed with PARP-2 antibody at a dilution of 1:1000.

Product Information

Tested Applications ChIP, IF, IP, WB

Applications Validated by Active Motif: ChIP: 2 - 10 ul per ChIP IP: 2 - 10 ul per IP WB: 1:500 dilution The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.
Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen This PARP-2 antibody was raised against a recombinant protein corresponding to the C-terminal region of human PARP-2.
Full Name PARP-2 antibody (pAb)
Synonyms PARP-2, poly ADP-ribosyl transferase, PPOL, DNA damage, antibody, antibodies, polyclonal, apotosis, chip, chromatin immunoprecipitation, sample
Molecular weight 66 kDa
GenBank accession numberNP_005475
RRIDAB_2793328
Purification Method Affinity Purified
Buffer PBS, 30% glycerol, and 0.035% sodium azide.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

PARP-2 encodes a chromatin-associated enzyme, a poly-ADP ribosyl transferase, which modifies various nuclear proteins by poly-ADP ribosylation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation and tumor transformation. It also plays a role in the regulation of the molecular events involved in the recovery of cells from DNA damage. PARP-2 is cleaved by caspase enzymes during the process of apoptosis.