PARP-2 antibody (pAb)
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
ChIP, IF, IP, WB
Cat No : 39044,39743,39744 39743
Synonyms
Validation Data Gallery
Product Information
Tested Applications |
ChIP, IF, IP, WB
Applications Validated by Active Motif: ChIP: 2 - 10 ul per ChIP IP: 2 - 10 ul per IP WB: 1:500 dilution The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required. |
Tested Reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | This PARP-2 antibody was raised against a recombinant protein corresponding to the C-terminal region of human PARP-2. |
Full Name | PARP-2 antibody (pAb) |
Synonyms | PARP-2, poly ADP-ribosyl transferase, PPOL, DNA damage, antibody, antibodies, polyclonal, apotosis, chip, chromatin immunoprecipitation, sample |
Molecular weight | 66 kDa |
GenBank accession number | NP_005475 |
RRID | AB_2793328 |
Purification Method | Affinity Purified |
Buffer | PBS, 30% glycerol, and 0.035% sodium azide. |
Storage | Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage. |
Background Information
PARP-2 encodes a chromatin-associated enzyme, a poly-ADP ribosyl transferase, which modifies various nuclear proteins by poly-ADP ribosylation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation and tumor transformation. It also plays a role in the regulation of the molecular events involved in the recovery of cells from DNA damage. PARP-2 is cleaved by caspase enzymes during the process of apoptosis.