PARP-1 N-terminal antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

ChIP-Seq, CUT&Tag, IP, WB

Cat No : 61639,61640

Synonyms

PARP-1 N-terminal, ADPRT, poly ADP-ribosyl transferase, PPOL, pADPRT-1, DNA damage, antibody, antibodies, polyclonal, parp, parp1, DNA damage, ribosylation, adp-ribosylation, apoptosis, pAb, polyclonal, ADPRT, poly ADP-ribosyl transferase, western blotting, wb, immunopreciptiation, ip, antibody, antibodies, sample

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Validation Data Gallery

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  PARP-1 N-terminal antibody (pAb) antibody (rAb) tested by ChIP-Seq. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 30 ug of chromatin from human NCI-H209 lung cancer cells and 5 ug of PARP-1 N-terminal antibody (pAb). ChIP DNA was sequenced on the Illumina NextSeq and 7.7 million sequence tags were mapped to identify PARP binding sites on chromosome 2.

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  PARP-1 N-terminal antibody (pAb) tested by Immunoprecipitation. 10 ug of PARP-1 antibody was used to immunoprecipitate PARP-1 from 250 ug of HeLa nuclear cell extract (lane 2). 10 ug of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the PARP-1 antibody at a dilution of 1 ug/ml.

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  PARP-1 N-terminal antibody (pAb) tested by Western blot. HeLa nuclear extract (25 ug) was probed with PARP-1 N-terminal antibody at a dilution of 1 ug/ml.

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Product Information

• Tested Applications: ChIP-Seq, CUT&Tag, IP, WB

  Applications Validated by Active Motif: ChIP-Seq: 5 ug per ChIP IP: 10 ug per IP WB*: 0.2 - 1 ug/ml dilution CUT&Tag: 1 ul per 50 ul reaction *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: This antibody was raised against a His-Tagged fusion protein corresponding to the N-terminal half of human PARP-1.
• Full Name: PARP-1 N-terminal antibody (pAb)
• Synonyms: PARP-1 N-terminal, ADPRT, poly ADP-ribosyl transferase, PPOL, pADPRT-1, DNA damage, antibody, antibodies, polyclonal, parp, parp1, DNA damage, ribosylation, adp-ribosylation, apoptosis, pAb, polyclonal, ADPRT, poly ADP-ribosyl transferase, western blotting, wb, immunopreciptiation, ip, antibody, antibodies, sample
• Molecular weight: 120 kDa
• GenBank accession number: NP_001609
• RRID: AB_2793715
• Purification Method: Protein A Chromatography
• Buffer: Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, a sera version (Catalog No. 39559) of this antibody is also available.
• Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

PARP-1 N-terminal (ADPRT) encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, that modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation and tumor transformation. It also plays a role in the regulation of the molecular events involved in the recovery of cells from DNA damage. Cleavage of PARP-1 (ADPRT) occurs following caspase activation during apoptosis. For additional information on PARP-1, please see the review article PARP-1: An Abundant and Ubiquitous Protein with Roles in Many Cellular Processes in the Targets & Applications section of our website.