PARP-1 C-terminal antibody (pAb)

Host / Isotype

Rabbit / Serum

Reactivity

Human

Applications

ChIP, ChIP-Seq, WB

Cat No : 39561,39562

Synonyms

parp, parp1, DNA damage, ribosylation, adp-ribosylation, apoptosis, pAb, polyclonal, ADPRT, poly ADP-ribosyl transferase, antibody, antibodies, sample

---

Validation Data Gallery

• 

  PARP-1 C-terminal antibody (pAb) antibody (rAb) tested by ChIP-Seq.Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 30 ug of chromatin from human NCI-H209 lung cancer cells and 10 ul of PARP-1 C-terminal antibody (pAb). ChIP DNA was sequenced on the Illumina NextSeq and 7.7 million sequence tags were mapped to identify PARP binding sites on chromosome 17.

• 

  PARP-1 C-terminal antibody tested by ChIP analysis. Chromatin IP performed using the ChIP-IT Express Kit (Catalog No. 53008) and MCF-7 chromatin (1.5 x 106 cell equivalents per ChIP) using 10 ul of PARP-1 C-terminal antibody or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the BRCA2 gene promoter. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.

• 

  PARP-1 C-terminal antibody tested by Western blot. HeLa nuclear extract (20 ug per lane) was probed with PARP-1 C-terminal antibody at a dilution of 1:6,000.

---

Product Information

• Tested Applications: ChIP, ChIP-Seq, WB

  Applications Validated by Active Motif: ChIP: 10 ul per ChIP WB: 1:1,000 - 1:6,000 dilution

• Tested Reactivity: Human
• Host / Isotype: Rabbit / Serum
• Class: Polyclonal
• Type: Antibody
• Immunogen: This PARP-1 C-terminal antibody was raised against a His-Tagged fusion protein corresponding to the C-terminal half of human PARP-1 was used to generate this PARP-1 antibody.
• Full Name: PARP-1 C-terminal antibody (pAb)
• Synonyms: parp, parp1, DNA damage, ribosylation, adp-ribosylation, apoptosis, pAb, polyclonal, ADPRT, poly ADP-ribosyl transferase, antibody, antibodies, sample
• Molecular weight: 120 kDa
• GenBank accession number: NP_001609
• RRID: AB_2793258
• Purification Method: None
• Buffer: Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
• Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

PARP-1 C-terminal (ADPRT) encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, that modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation and tumor transformation. It also plays a role in the regulation of the molecular events involved in the recovery of cells from DNA damage. Cleavage of PARP-1 (ADPRT) occurs following caspase activation during apoptosis. For additional information on PARP-1, please see the review article PARP-1: An Abundant and Ubiquitous Protein with Roles in Many Cellular Processes in the Targets & Applications section or our website.