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OGT/O-GlcNAc transferase antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

ChIP, ChIP-Seq, WB

Activ Motif logo

Cat No : 61355,61356 61355

Synonyms

OGT, OGT/O-GlcNAc, OGT/O-GlcNAc transferase, O-GlcNAc transferase, O-linked N-acetylglucosamine (GlcNAc) transferase, (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase), O-GlcNAc transferase p110 subunit2, HRNT1, UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit, OGLCNAC, uridinediphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyl transferase, O-GlcNAc transferase subunit p110, O-linked N-acetylglucosamine transferase 110 kDa subunit, antibody, polyclonal, pAb, sample, western blotting

Validation Data Gallery

  • OGT/O-GlcNAc transferase (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with chromatin from 3 million primary mouse T cells and 5 ul of OGT antibody. ChIP DNA was sequenced on the Illumina HiSeq and 14 million sequence tags were mapped to identify OGT binding sites. The image on the left shows many OGT binding sites within a 25 million bp region on mouse chromosome 8. The image on the right shows that binding sites are localized to gene promoters as expected based on the known interaction with the H3K4 methylating COMPASS complex.

    OGT/O-GlcNAc transferase (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with chromatin from 3 million primary mouse T cells and 5 ul of OGT antibody. ChIP DNA was sequenced on the Illumina HiSeq and 14 million sequence tags were mapped to identify OGT binding sites. The image on the left shows many OGT binding sites within a 25 million bp region on mouse chromosome 8. The image on the right shows that binding sites are localized to gene promoters as expected based on the known interaction with the H3K4 methylating COMPASS complex.

  • OGT/O-GlcNAc transferase (pAb) tested by Western blot. Detection of OGT/O-GlcNAc transferase by Western blot. The analysis was performed using 20 ug HeLa whole-cell extract and OGT/O-GlcNAc transferase (pAb) at a 1:500 dilution.

    OGT/O-GlcNAc transferase (pAb) tested by Western blot. Detection of OGT/O-GlcNAc transferase by Western blot. The analysis was performed using 20 ug HeLa whole-cell extract and OGT/O-GlcNAc transferase (pAb) at a 1:500 dilution.

Product Information

Tested Applications ChIP, ChIP-Seq, WB

Applications Validated by Active Motif: ChIP: 5 ul per ChIP ChIP-Seq & ChIP-chip: 5 ul each WB: 1:500- 1:2,000 dilution
Tested Reactivity Human, Mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
ModificationAcetylated
Immunogen This antibody was raised against a recombinant protein corresponding to the N-terminal region of human OGT.
Full Name OGT/O-GlcNAc transferase antibody (pAb)
Synonyms OGT, OGT/O-GlcNAc, OGT/O-GlcNAc transferase, O-GlcNAc transferase, O-linked N-acetylglucosamine (GlcNAc) transferase, (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase), O-GlcNAc transferase p110 subunit2, HRNT1, UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit, OGLCNAC, uridinediphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyl transferase, O-GlcNAc transferase subunit p110, O-linked N-acetylglucosamine transferase 110 kDa subunit, antibody, polyclonal, pAb, sample, western blotting
Molecular weight 120 kDa
GenBank accession numberNP_858058
RRIDAB_2793604
Purification Method Affinity Purified
Buffer Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

OGT (O-linked N-acetylglucosamine (GlcNAc) transferase) catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to serine or threonine residues in cytoplasmic and nuclear proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc). OGT can glycosylate a large and diverse number of proteins including HCF1, AKT1, MLL5 and histone H2B. It can regulate their cellular processes via cross-talk between glycosylation and phosphorylation or by affecting proteolytic processing. Specifically OGT has been shown to interact directly with TET2 and TET3, enzymes that catalyze the oxidation of 5-methylcytosine on DNA. The TET/OGT interaction leads to GlcNAcylation of HCF1, a protein component of the COMPASS complex, which regulates H3K4 methylation and gene expression.